Isolation,characterization and extraction of mer gene of Hg²⁺ resisting strain D₂

【摘要】 Mercury-resistant strain D2 was isolated from mercury-contaminated soil and investigations on its 16S rDNA sequence,growth,minimal inhibitory concentrations(MICs) of metals,antibiotic susceptibility and mer gene were conducted.The strain D2 can grow in the medium containing 60 mg/L mercury ion.It presents more than 99% identity of 16S rRNA gene with Pseudomonas aeruginosa,and exhibits high MIC values for heavy metals and a large spectrum antibiotics resistance.The mer RT gene sequence was amplified from chromosome.Strain D2 is identified as Pseudomonas aeruginosa and the resistance to mercuric ion is related to chromosome.更多还原

【Abstract】 Mercury-resistant strain D2 was isolated from mercury-contaminated soil and investigations on its 16S rDNA sequence,growth,minimal inhibitory concentrations(MICs) of metals,antibiotic susceptibility and mer gene were conducted.The strain D2 can grow in the medium containing 60 mg/L mercury ion.It presents more than 99% identity of 16S rRNA gene with Pseudomonas aeruginosa,and exhibits high MIC values for heavy metals and a large spectrum antibiotics resistance.The mer RT gene sequence was amplified from chromosome.Strain D2 is identified as Pseudomonas aeruginosa and the resistance to mercuric ion is related to chromosome.更多还原