【摘要】 目的:研究二甲氧雌二醇（2-ME）对人白血病细胞K562细胞的增殖、凋亡的作用及其机制。方法:分别以不同浓度的2-ME处理白血病细胞K562,通过MTT法检测K562细胞活力,应用Annexin V和PI双染的流式细胞术检测K562细胞凋亡率,应用凝胶蛋白电泳迁移率分析法EMSA检测K562细胞核内NF-KB蛋白的结合活性变化情况。结果:随着2-ME浓度的升高,各项指标与对照组相比较的差异均有统计学意义（P<0.01）;K562细胞增殖明显受到抑制;细胞凋亡率明显增加,且当2-ME浓度为8μmol·L^-1时,细胞凋亡率达64.3%;同时K562细胞核内NF-kappa B的DNA结合活性明显降低。结论:2-ME可显著抑制人白血病细胞K562增殖并诱导其凋亡;2-ME诱导K562细胞凋亡的机制与NF-kappa B蛋白信号通路有关。更多还原

【Abstract】 Objective:To study the effect and mechanism of 2-methoxyestradiol(2-ME) on proliferation,apoptosis of leukemia K562.Method:K562 cells were treated with 2-ME of different concentrations in vitro.Cell proliferation inhibition was detected by MTT colorimetric assay;Cell apoptosis rate were measured by flow cytometry(FCM).Activity of NF-kappa B in nucleus were detected by electrophoretic mobility shift assay(EMSA) Result:2-ME was inhibited significandy the growth of leukemia K562 cells in a time-dependent and dose-dependent manner(P<0.01),after treatment with 2-ME,K562 cells apoptotic rate was increased significantly and activity of NF-kappa B in nucleus were significantly lower.Conclusion:The 2-ME inhibits the proliferation,induces apoptosis of K562 cells via inhibit the activity of NF-kappa B in nucleus.This study provides useful experimental data for clinical application of 2-ME.更多还原