【摘要】 目的:建立HPLC法测定盐酸美沙酮口服溶液含量。方法:采用ZORBAX SB-C18(4.6×250mm,5μm)色谱柱,以乙腈-磷酸盐(PH2.5)=32:68(v/v)为流动相,流速:1.5mL.min-1,检测波长:220nm。结果:盐酸美沙酮在10-100μg.ml-1线性关系良好(r=0.9998)。盐酸美沙酮口服溶液平均回收率分别为99.80%,99.75%,99.76%(RSD0.28%,0.34%,0.20%)。结论:该法操作简便、结果准确可靠、精密度好,可用于盐酸美沙酮口服液的含量测定。更多还原

【Abstract】 Objective:To establish the HPLC Determination of methadone hydrochloride oral solution content.Method:A column of ZORBAX SB-C18 (4.6 × 250mm,5μm),acetonitrile-phosphate (PH2.5)=32:68 (v /v) as mobile phase,flow rate:1.5 ml o min-1,The detection wave length:220nm.Results:methadone hydrochloride was a good linear relationship in 10-100μg /ml (r=0.9998).the method recovery of methadone hydrochloride Oral solution was 99.80%,99.75%,99.76% (RSD0.28%,0.34%,0.20%).Conclusion:The method is simple and precise,the result is accurate and reliable,and can be used for the determination of methadone hydrochloride oral solution content.更多还原