【摘要】 目的分离培养患者粪便标本中的霍乱弧菌,对其进行种属鉴定和毒力基因及药物敏感性检测。方法常规方法分离菌株,根据其生化及血清学反应结果鉴定菌株,聚合酶链反应检测霍乱肠毒素基因(ctxAB),K-B法分析药物敏感性。结果从患者粪便中分离到O139霍乱弧菌1株,携带霍乱肠毒素基因(ctxAB),对头孢他啶、头孢哌酮等常用药物敏感,对氯霉素、呋喃妥因、庆大霉素、四环素、氨苄西林耐药。结论该株菌为霍乱弧菌流行株,聚合酶链反应可较快完成对霍乱弧菌肠毒素基因(ctxAB)的检测。在对O139群霍乱弧菌引起的霍乱疫情防控工作中,应密切监测其耐药性和毒力基因携带情况,为临床有针对性用药提供依据,及时控制疫情。更多还原

【Abstract】 [Objective]To isolate and culture vibrio cholerae in patients’ stool specimens,and perform species identification and virulence genes detection and drug sensitivity test on it.[Methods]Conventional method was used for isolation,and the identification was based on the biochemical and serological results,polymerase chain reaction for detection of cholera toxin gene (ctxAB),K-B method for drug sensitivity test.[Results]One strain of vibro cholerae O139,carrying cholera toxin gene (ctxAB),was isolated from patient’s stool,and sensitive to ceftazidime,cefoperazone and other commonly used drug,but resistant to chloramphenicol,nitrofurantoin,gentamicin,tetracycline,ampicillin.[Conclusion]This isolate is epidemic strain of vibrio cholerae,polymerase chain reaction can perform ctxAB detection fast.In the prevention of cholera epidemic caused by vibro cholerae O139,its drug resistance and carrying status of toxin gene should be closely monitored to provide evidence for clinical drug use and timely control of outbreaks.更多还原