【摘要】 目的:探讨榄香烯(elemene,Ele)抑制尾加压素Ⅱ(Urotensin Ⅱ,U-Ⅱ)诱导的人晶状体上皮细胞(hu-man lens epithelial cell,HLEC)增殖及信号转导机制。方法:将U-Ⅱ诱导体外培养的HLEC发生增殖,并用不同浓度的Ele与其共同孵育后,用四甲基偶氮唑蓝(methyl thiazolyl tetrazolium,MTT)法检测HLEC的活性;用流式细胞术(flow cytometer,FCM)检测HLEC增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)表达;用荧光分光光度法检测HLEC内游离钙离子浓度[Ca2+]i;用放射免疫分析法检测HLEC内环化腺苷酸(cyclicadenosine monophosphate,cAMP)和环化鸟苷酸(cyclic guanosine monophosphate,cGMP)浓度。结果:U-II组HLEC活性、PCNA蛋白表达和cGMP浓度均高于对照组(P<0.001);Ele组HLEC活性、PCNA蛋白表达和cGMP浓度均比U-II组降低(P<0.001)。U-II组HLEC[Ca2+]i比对照组显著升高(P<0.001);Ele组与U-II组比较[Ca2+]i也显著升高(P<0.001)。U-II组cAMP浓度比对照组显著降低(P<0.001),Ele组cAMP浓度与U-II组比较显著升高(P<0.001)。结论:Ele可抑制U-II诱导的HLEC增殖。[Ca2+]、cAMP-PKA、cGMP-PKG是其重要的细胞信号转导机制。Ele有可能成为防治后发性白内障的有效药物。更多还原

【Abstract】 Objective: To study the inhibitory effect of elemene( Ele) on proliferation induced by urotensin Ⅱ in human lens epithelial cell( HLEC) and the mechanism of signal transduction. Methods: The proliferations of cultured HLEC were induced by urotensin Ⅱ( U Ⅱ) ,which were incubated with different concentrations of Ele. The HLEC activities were detected via methyl thiazolyl tetrazolium(MTT) . The proliferating cell nuclear antigen( PCNA) of HLEC were detected via flow cytometer( FCM) heconcentration of intracellular free calcium( [Ca2 + ]i) of HLEC were determined with Fura 2 /AM by spectrofluoremeter. The intracellular contents of cyclic adenosine monophosphate (cAMP ) and cyclic guanosine monophosphate( cGMP) of HLEC were assayed by radioimmunoassay. Results: The activity,expression of PCNA and the cGMP concentration of HLEC in U II group were higher than that in control group,there were decreased in Ele group significantly( P < 0. 001) . The [Ca2 + ]i concentration of HLEC in U Ⅱ group were higher than that in control group,there were increased in Ele group significantly ( P < 0. 001) . The cAMP concentration of HLEC in U II group were decreased to compare with control group,but there were increased in Ele group significantly( P < 0. 001) . Conclusion: Ele can inhibit the proliferation of HLEC induced by urotensin II. [Ca2 + ],cAMP PKA and cGMP PKG were the important mechanism of signal transduction. Ele may become an effective drug of preventing and treating after cataract.更多还原