【摘要】 目的:测定不同采收时期野老鹳草中活性成分没食子酸及鞣花酸的含量,为最佳采收期的确定提供依据。方法:采用HPLC对不同采收期的野老鹳草中没食子酸及鞣花酸的含量进行测定。色谱条件:色谱柱为Dia-monsil C18色谱柱(150 mm×4.6 mm,5μm);流动相为乙腈-0.1%磷酸溶液梯度洗脱;流速为1.0 mL/min;检测波长为274 nm;柱温为25℃。结果:没食子酸及鞣花酸的线性范围分别是:0.075～5.00μg(r=0.9995)、0.05～2.00μg(r=0.9995),平均回收率分别是99.88%(RSD=1.19%)、99.08%(RSD=2.81%)。结论:野老鹳草中没食子酸及鞣花酸的含量均在开花期开始上升,于始果期达到最高。更多还原

【Abstract】 Objective: To determine the contents of the active components,gallic acid and ellagic acid in Geranium carolinianum from different collection time and to define the best collection time for this herb.Methods: The contents of gallic acid and ellagic acid in each samples of Geranium carolinianum were determined by HPLC.The HPLC method was performed on a Diamonsil C18 column(150 mm × 4.6 mm,5 μm) with acetonitrile-0.1% H3PO4 as mobile phase.The flow rate was 1.0 mL/min,the detection wavelength was 274 nm and the column temperature was 25 ℃.Results: The calibration curve of gallic acid and ellagic acid were linear in the range of 0.075 ～ 5.00 μg(r = 0.9995) and 0.05 ～ 2.00 μg(r = 0.9995),respectively.The average recovery of gallic acid and ellagic acid were 99.88%(RSD = 1.19%) and 99.08%(RSD = 2.81%),respectively.Conclusion: The content of gallic acid and ellagic acid in Geranium carolinianum both began to increase in flowering stage and increased to the maximum value in immature-fruit stage.更多还原