【摘要】 目的:首次对麻花秦艽的染色体数目、核型等进行分析研究,并与秦艽和小秦艽进行比较,为其遗传和进化提供了进一步的细胞学证据。方法:根尖用0.002mol/L的8-羟基喹啉溶液预处理5.7h,用2.5%混合酶液,于25℃下酶解1.6h,低渗处理3h(置于冰箱内),然后采用空气干燥制片法,结合显微摄影对染色体进行分析。结果:麻花秦艽核型公式是K(2n)=26=2M+24m,核型不对称系数(AS.K)为52.68%,属于"1A"型。结论:麻花秦艽的核型与秦艽、小秦艽相比较,秦艽最为进化,麻花秦艽介于秦艽和小秦艽之间。更多还原

【Abstract】 Objective:Study on chromosome number, karyotype of Gentiana straminea for the first time. Compare karyotypes of Gentiana straminea, Gentiana macrophylla and Gentiana dahurica. Provide cytological evidence for further studies on genetics and evolution. Methods: Soak the root tip in 0.002 mol/L 8-oxychinolin solution for 5.7 h. Decomposed in 2.5% mixed enzyme solution for 1.6 h at 25 ℃ and use Hypotonic treatment for 3 h in refrigerator. At last, make specimen slides by the Air-drying technique. Sections combined with micrograph were used to analyze chromosome. Results:The karyotypes formula of Gentiana straminea is K(2n)=26=2M+24m, the AS.K was 52.68%, which belong to "1A" type. Conclution:Compare karyotypes of Gentiana straminea, Gentiana macrophylla and Gentiana dahurica, the result showed that Gentiana macrophylla lives in highest stage of evolution. Gentiana straminea is intermediate between Gentiana macrophylla and Gentiana dahurica.更多还原