Shenmai Injection(参麦注射液) Inhibiting the Extracellular Signal Regulated Kinase-lnduced Human Airway Smooth Muscle Proliferation in Asthma

【摘要】 <正>Objective:To investigate the relationship between the proliferation of sensitized human airway smooth muscle cells（HASMCs）and the expression of extracellular signal regulated kinase（ERK）and the effect of Shenmai Injection（参麦注射液,SMI）on HASMCs.Methods:The HASMCs cultured in vitro were divided into three groups:（1）control group;（2）sensitized group:containing 10%asthmatic serum;（3）SMI group:further divided into three different concentration subgroups interferred with 10μL/mL,50μL/mL,and 100μL/mL SMI, respectively.The proliferation of HASMCs was detected using MTT method,the expression of proliferating cell nucleus antigen（PCNA）in HASMCs was detected using immunocytochemical staining,and the expression of phosphoration-ERK1/2（p-ERK1/2）protein was detected using Western-blot.Results:After passive sensitization, the optical density value(A₄₉₀value)of HASMCs was significantly increased from 0.366±0.086 to 0.839±0.168（P<0.05）.In addition,the expression of PCNA was significantly increased from 28.7%±5.9%in the control group to 69.8%±7.5%in the sensitized group（P<0.05）.At the same time,the expression of p-ERK1/2 in passively sensitized HASMCs was significantly increased compared with the control group（all P<0.05）.After application of 10μL/mL,50μL/mL,and 100μL/mL SMI to the cultured media of passively sensitized group,the A₅₇₀value was significantly decreased from 0.839±0.168 to 0.612±0.100,0.412±0.092,and 0.339±0.077, respectively（P<0.05）.Moreover,the expression of PCNA was significantly decreased from 69.8%±7.5%to 57.8%±6.2%,40.7%±5.4%,and 26.1%±5.2%,respectively.At the same time,the expression of p-ERK1/2 in each SMI group was significantly decreased compared with the sensitized group（ail P<0.05）.Conclusion:ERK signal transduction pathway may be involved in the airway remodeling in asthma.The expression of ERK can be inhibited by SMI in a dose-dependent manner,thus preventing the proliferation of HASMCs.更多还原

【Abstract】 Objective:To investigate the relationship between the proliferation of sensitized human airway smooth muscle cells（HASMCs）and the expression of extracellular signal regulated kinase（ERK）and the effect of Shenmai Injection（参麦注射液,SMI）on HASMCs.Methods:The HASMCs cultured in vitro were divided into three groups:（1）control group;（2）sensitized group:containing 10%asthmatic serum;（3）SMI group:further divided into three different concentration subgroups interferred with 10μL/mL,50μL/mL,and 100μL/mL SMI, respectively.The proliferation of HASMCs was detected using MTT method,the expression of proliferating cell nucleus antigen（PCNA）in HASMCs was detected using immunocytochemical staining,and the expression of phosphoration-ERK1/2（p-ERK1/2）protein was detected using Western-blot.Results:After passive sensitization, the optical density value(A₄₉₀value)of HASMCs was significantly increased from 0.366±0.086 to 0.839±0.168（P<0.05）.In addition,the expression of PCNA was significantly increased from 28.7%±5.9%in the control group to 69.8%±7.5%in the sensitized group（P<0.05）.At the same time,the expression of p-ERK1/2 in passively sensitized HASMCs was significantly increased compared with the control group（all P<0.05）.After application of 10μL/mL,50μL/mL,and 100μL/mL SMI to the cultured media of passively sensitized group,the A₅₇₀value was significantly decreased from 0.839±0.168 to 0.612±0.100,0.412±0.092,and 0.339±0.077, respectively（P<0.05）.Moreover,the expression of PCNA was significantly decreased from 69.8%±7.5%to 57.8%±6.2%,40.7%±5.4%,and 26.1%±5.2%,respectively.At the same time,the expression of p-ERK1/2 in each SMI group was significantly decreased compared with the sensitized group（ail P<0.05）.Conclusion:ERK signal transduction pathway may be involved in the airway remodeling in asthma.The expression of ERK can be inhibited by SMI in a dose-dependent manner,thus preventing the proliferation of HASMCs.更多还原