【摘要】 目的:探讨冠心病(coronary heart disease,CHD)患者焦虑情绪与载脂蛋白E(ApoE)基因多态性的关系。方法:对符合1979年世界卫生组织CHD诊断标准的107例冠心病患者,进行汉密顿焦虑量表(Hamilton Anxiety Scale,HAMA)、焦虑自评量表(Self-Rating Anxiety Scale,SAS)测评,依焦虑评分情况将患者分为CHD伴焦虑组(焦虑组)、CHD不伴焦虑组(无焦虑组),选择53例健康人为对照组。于入院2日内留存血样,以Hhal限制性内切酶酶切及琼脂糖凝胶电泳确定ApoE基因多态性,采用生物化学方法测定血清血脂。结果:焦虑组ε4等位基因频率高于无焦虑组和对照组(26.0%vs.10.4%,26.0%vs.13.6%;P=0.021,0.004)。焦虑组E4/4基因型频数高于无焦虑组(4vs.0,P=0.032)。焦虑组E3/4基因型频数高于对照组(17vs.8,P=0.018)。无焦虑组ε4等位基因频率高于对照组,但差异无统计学意义(13.6%vs.10.4%,P=0.802)。CHD组携带ε4等位基因患者HAMA[(13.2±5.2)vs.(9.2±6.5)]、SAS评分[(42.3±7.7)vs.(37.1±8.3)]及总胆固醇[(5.4±1.1)mmol/Lvs.(4.8±1.3)mmol/L]、低密度脂蛋白水平[(3.6±1.0)mmol/Lvs.(3.1±1.1)mmol/L]均高于非携带ε4等位基因者(P<0.05)。结论:冠心病患者的焦虑情绪可能与ApoE基因多态性具有一定关系。ApoEε4等位基因可能是CHD患者焦虑情绪的危险因素。更多还原

【Abstract】 Objective:To investigate the genetic susceptibility and the relationship between apolipoprotein E(APOE)genetype and the anxiety symptoms in patients with coronary heart disease(CHD).Methods:The Hamilton Anxiety Scale(HAMA)and Self-rating Anxiety Scale(SAS)were applied to assess the anxiety symptoms of 107 CHD patients.According to the HAMA or SAS score,all patients were divided into CHD with anxiety symptom group(n=48),CHD without anxiety symptom group(n=59).And 53 normal persons were selected as controls.The genotypes and alleles of apolipoprotein E gene were determined with polymerase reaction(PCR)and restriction fragment length polymorphisms(RFLP)technique.The total serum cholesterol,lipoprotein were detected by biochemtry methods.Results:The frequency of APOE epsilon 4 in CHD patients with anxiety was significantly higher than that in CHD patients without anxiety(26.0% vs.13.6%,P=0.004)and normal controls(26.0% vs.10.4%,P=0.021).The frequency distribution of E4/4 genetype in CHD patients with anxiety was higher than that in patients without anxiety(4 vs.0,P=0.032),and the frequency distribution of E3/4 genetype in CHD patients with anxiety was higher than that in normal controls(17 vs.8,P=0.018).The frequency of epsilon 4 was higher in CHD patients without anxiety than that in control group,but there was no remarkable difference between two groups(13.6% vs.10.4%,P=0.802).Compared with CHD patients without epsilon 4,the HAMA score[(13.2±5.2)vs.(9.2±6.5)],SAS score[(42.3±7.7)vs.(37.1±8.3)],and the level of total cholesterol[(5.4±1.1)mmol/L vs.(4.8±1.3)mmol/L],low density lipoprotein[(3.6±1.0)mmol/L vs.(3.1±1.1)mmol/L]were higher in CHD patients with epsilon 4(all P<0.05).Conclusion:The results suggest that the polymorphisms of apolipoprotein E gene is associated with the anxiety symptoms in coronary heart disease.The apolipoprotein E epsilon 4 may be a risking factor for anxiety symptom in patients with coronary heart disease.更多还原