【摘要】 [目的]研究溃疡性结肠炎(UC)结肠黏膜中的黏蛋白MUC2、MUC5AC以及肠三叶因子(TFF3)的表达规律及其与中医证型的关系。[方法]选择UC患者74例,其中大肠湿热证组48例,脾胃气虚证组26例,另外选10例健康体检人群为正常对照组。通过免疫组织化学染色法测定3组人群肠黏膜上皮中MUC2、MUC5AC以及TFF3的表达情况。[结果]MUC2在UC大肠湿热组、脾胃气虚组患者表达较正常对照组减少,但差异无统计学意义(P>0.05)。大肠湿热组、脾胃气虚组MUC5AC的阳性表达率分别为33.3%和23.1%,正常对照组均为阴性,大肠湿热组MUC5AC阳性表达率与正常对照组相比差异有统计学意义(P<0.05),而脾胃气虚组与正常对照组对比差异无统计学意义。大肠湿热组TFF3阳性表达率为79.2%,高于脾胃气虚组的53.9%,两者的阳性表达与正常对照组相比,均差异有统计学意义(P<0.05)。脾胃气虚组与大肠湿热组间MUC2、MUC5AC以及TFF3的表达均差异无统计学意义(P>0.05)。[结论]UC大肠湿热证MUC5AC的表达上调和TFF3的表达下调可能与UC的发生、发展有关,而且对临床诊疗、判断预后也有较大意义。更多还原

【Abstract】 [Objective]To study the expression pattern of mucin and TFF3 in the colonic mucosa of ulcerative colitis(UC)patients and their relationships with TCM(Traditional Chinese Medicine)syndrome type.[Methods]Among 74 cases of ulcerative colitis,48 cases were diagnosed as large intestine dampness-heat(LIDH)syndrome,and 26 cases as spleen-stomach Qi deficiency(SSQD)syndrome.Ten healthy people were set as control group.Immunohistochemical method was applied to detect the expression of MUC2,MUC5AC and TFF3 in the colonic mucosa of patients with different syndrome types.[Results]The positive expression rate of MUC2 in the colonic mucosa of LIDH and SSQD group was lower than that of control group,with no significant difference(P>0.05).The positive expression rate of MUC5AC in the colonic mucosa of LIDH and SSQD group were 33.3% and 23.1% respectively,and that of the normal control group was negative.MUC5AC expression in LIDH group was significantly higher than that of the normal control group(P<0.05).The expression rate of TFF3 was 79.2% in LIDH group,which was higher than SSQD group(53.9%).The expressions of MUC2,MUC5AC and TFF3 showed no significant difference between LIDH and SSQD group(P>0.05).[Conclusion]The up-regulation of MUC5AC expression and down-regulation of TFF3 expression may be involved in genesis and development of UC in patients with LIDH,which is valuable for UC clinical diagnosis and predicting the patient’s prognosis.更多还原