【摘要】 目的:研究针对人caspase-8基因mRNA的siRNA(small interfering RNA)表达载体,观察其转染人肝癌细胞HepG2后,细胞caspase-8基因表达的变化,及其对细胞凋亡的影响。方法:采用针对人caspase-8基因mRNA的siRNA表达质粒,由脂质体介导瞬时转染HepG2细胞株,并采用脂多糖(LPS)诱导HepG2细胞凋亡,RT-PCR、Westernblot测定caspase-8表达。结果:针对人caspase-8基因mRNA的siRNA表达质粒能抑制caspase-8mRNA和蛋白表达;转染后HepG2细胞体外生长明显受到抑制。结论:siRNA表达质粒能有效地抑制HepG2细胞中caspase-8的表达,抑制细胞凋亡并促进生长。为进一步探究肝衰竭基因治疗提供了实验依据。更多还原

【Abstract】 Objective:To investigate the effects of siRNA targeting casepase-8 gene and to observe its influence on apoptosis of HepG2 cells after transfected into cells.Methods:siRNA were transiently transfected into HepG2 cells via cathodolyte liposome transfectiojn method.After induced with LPS,the interference effect was detected by RT-PCR and Western blot.Results:siRNA could restrain the casepase-8 mRNA and protein express,and restrain the cells graning.Conclusion:The constructed siRNA expressing plasmid can reduce the expression of caspase-8,inhibit the proliferation of HepG2 and induce apoptosis.The experiment provides the foundation for gene therapy of hepatic failure.更多还原