【摘要】 目的探讨热休克蛋白70(heat shock protein 70,HSP70)在重症急性胰腺炎(severe acute pancre-atitis,SAP)大鼠肺组织的变化及氯胺酮对其表达的影响。方法 48只健康雄性SD大鼠随机分为对照组、SAP组和氯胺酮干预组(KTM组),每组16只。应用逆行胰胆管穿刺注射5%牛磺胆酸钠诱导大鼠重症急性胰腺炎。氯胺酮干预组术后静脉给予50mg/(kg·h)氯胺酮。术后12h检测肺湿重系数,肺泡灌洗液中蛋白含量和细胞数及肺组织中HSP70的变化。结果与SAP组相比,氯胺酮干预组能明显提高SAP术后12h肺组织HSP70水平(P<0.05),并降低肺湿重系数、肺泡灌洗液中蛋白含量和细胞数水平(P<0.05)。结论 SAP肺损伤后HSP70明显增加,氯胺酮可能通过上调肺组织内HSP70的表达起到肺保护的作用。更多还原

【Abstract】 Objective To evaluate the alteration of heat shock protein 70 (HSP 70) of lung during severe acute pancreatitis (SAP) and the therapeutic effects of ketamine in rats. Methods A total of 48 SD male rats were randomly divided into Group A (sham-operative group), Group B (SAP group) and Group C (SAP treated with ketamine), 16 animals in each group. The model of rat SAP induced by retrograde injection of 5% sodium taurocholate into the pancreatic duct of rats. And the treatment groups received ketmine with doses of 50 mg/(kg·h) injected continually immediately after the preparation of SAP were made. All rats were killed at 12 hours after operation. Bronchoalveolar lavage fluid (BALF) was collected for determination of pro-tein and PMN count. The lungs were removed for evaluation of HSP 70 and determination of wetPdry lung weight ratio. Results In SAP with ketamin therapy group, the levels of HSP 70 were significantly higher than those of SAP group (P <0.05), and the wetPdry lung weight ratio and the protein and PMN count in BALF were significantly lower than SAP group. ConclusionHSP 70 are increased remarkably in SAP. Ketamine has protective effect against severe acute pancreatitis associated lung injury in rats by inducing the peak level of HSP 70.更多还原