节点文献

壳多糖对增殖的血管平滑肌细胞[Ca²⁺]i和[H⁺]i的影响

Effects of chitosan on [Ca²⁺]i and [H⁺]i of vascular smooth muscle cells

推荐 CAJ下载
PDF下载
不支持迅雷等下载工具，请取消加速工具后下载。

【Author】 WANG Da-xin1,LI hui1,LI Xue-ping2 (1.Department of Cardiovascular,Clinical Medical College of Yangzhou University,Medical College of Yangzhou University,Yangzhou,Jiangsu 225001,P.R.China; 2.Department of Rehabilitation Medicine,the Affiliated Nanjing First Hospital with Nanjing Medical University,Nanjing,Jiangsu 225001,P.R.China)

【机构】扬州大学临床医学院(扬州大学医学院)心脏科；南京医科大学附属南京第一医院康复医学科；

【摘要】目的探讨壳多糖对血管平滑肌细胞[Ca2+]i和[H+]i的影响。方法采用荧光探针标记法观察小牛血清对血管平滑肌细胞[Ca2+]i和[H+]i的影响,以及壳多糖对其的干预作用。结果当NBS刺激血管平滑肌细胞后[Ca2+]i浓度和细胞内pH均明显增加;而壳多糖与NBS合用时,胞内[Ca2+]i浓度和pH降低明显降低。结论壳多糖抑制小牛血清诱导的血管平滑肌细胞[Ca2+]i浓度增加和pH上升,说明壳多糖抑制血管平滑肌细胞增殖可能与抑制Ca2+动员有关。更多还原

【Abstract】【Objective】To study the effects of chitosan on [Ca2+]i and [H+]i of vascular smooth muscle cells (VSMC).【Methods】By means of fluorescence probe marking method,we observed the effect of newborn bull serum (NBS,20%) on VSMC [Ca2+]i and [H+]i,and intervention effect of chitosan on theirs.【Result】The [Ca2+]i concentration and pH all increased in cultured VSMC after NBS exposure. These effects were inhibited by chitosan (2000 μg/L).【Conclusion】Chitosan might inhibit Ca2+ mobilization,through which [Ca2+]i concentration and pH of VSMC were inhibited.更多还原