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制霉素搽剂微生物检查法的建立
Microbial limit test of nystatin liniment
【摘要】 目的建立制霉素搽剂微生物限度检查法。方法采用离心沉淀薄膜过滤法联用0.1%吐温80-pH7.0氯化钠-蛋白胨缓冲液作冲洗剂,测定金黄色葡萄球菌等5种试验菌回收率,对控制菌进行验证。结果试验菌回收率高于70%,控制菌试验组呈阳性反应,阴性菌对照组呈阴性反应。结论采用离心沉淀薄膜过滤法可以消除处方中不溶颗粒的干扰及制霉素在试验条件下的抑菌作用,顺利检出该品种所污染的各种微生物。
【Abstract】 Objective To establish a method of microbial limit test for nystatin liniment.Methods Centrifugation sedimentation combined with membrane filtration method and the 0.1% polysorbate 80-sodium chloride-peptone buffer were used to determine the recovery rate 5 kinds of microorganisms and verify the control bacteria.Results The recovery of bacteria,yeast and mould were higher than 70%.Control bacteria were positive and Gram-negative organisms were negative.Conclusion Centrifugation sedimentation combined with membrane filtration method can eliminate the interference of the insoluble particles and antibacterial activity of nystatin liniment.
【Key words】 Nystatin liniment; Microbial limit test; Centrifugation sedimentation membrane filtration method;
- 【文献出处】 中国现代药物应用 ,Chinese Journal of Modern Drug Application , 编辑部邮箱 ,2010年09期
- 【分类号】R927.2
- 【被引频次】3
- 【下载频次】67