【摘要】 以麻竹(Dendroc alamus latiflorus Munro)花药为材料,于M8+2mg·L-1NAA+0.5mg·L-16-BA+15mg·L-1PAA+7.5mg·L-1STS+500mg·L-1CH+100mg·L-1proline+100mg·L-1glutamin+5.4%maltose+0.8%agar的诱导培养基上成功诱导出胚性愈伤组织,在此培养基上继代可形成体胚并分化成苗,初步建立了麻竹花药一步成苗的再生体系。更多还原

【Abstract】 Embryogenic callus was initiated from bamboo (Dendrocalamus latiflorus Munro) anthers cultured on M8 medium supplemented with 2 mg·L-1 NAA,0.5 mg·L-1 6-BA,15 mg·L-1 phenylacetic acid (PAA),7.5 mg·L-1 silver thiosulfate (STS),500 mg·L-1 casein enzymatic hydrolysate (CH),100 mg·L-1 proline,100 mg·L-1 glutamine,5.4% maltose,and 0.8% agar.Subculture of these embryogenic calli on the same medium resulted in embryoid formation and their subsequent germination to form rooted plantlets.A one-step method for anther culture and plant regeneration of D.latiflorus was preliminarily established.更多还原