【摘要】 目的:建立食品中硒元素的原子荧光光度测定方法。方法:样品经过酸加热消化后,用硼氢化钠作还原剂,还原成硒化氢(SeH2),由载气带入原子化器中进行原子化,在硒特制空心阴极灯照射下,基态硒原子被激发至高能态,在去活化回到基态时,发射出特征波长的荧光,其荧光强度与硒含量成正比。与标准系列比较定量。结果:硒的浓度在1.0μg/L～20.0μg/L之间线性关系良好,相关系数r=1.0000,回归方程y=177.421x-11.782。结论:原子荧光法测定硒的检出限可达ppb级,AFS9800的检出限0.0989μg/L。该法灵敏度高,准确度好,精密度高,线性范围宽,所用试剂毒性小,简便。因此可以广泛的应用于食品及水质中硒的测定。更多还原

【Abstract】 Objective:To establish a method for the determination of selenium in food by atomic fluorescence spectrometry.Methods:After the sample was digested by heating in acid,hydrogen selenide(SeH2) was generated using sodium borohydride as the reducer and introduced with the carrier into the atomizer for atomization.Under irradiation of a hollow cathode lamp of selenium,ground state selenium atoms were excited to high-energy state and,when returning to the ground state after deactivation,emitted fluorescence of characteristic wavelengths.The fluorescence intensity was positively proportional to the content of selenium and,based on this,quantification was performed against a standard series.Results:Good linearity was presented within the selenium concentration range of 1.0 μg/L～20.0 μg/L;the regression equation was y=177.421x-11.782(r=1.0000).Conclusion:For determination of selenium by atomic fluorescence spectrometry,the limit of detection could be of as low as ppb grade;the limit of detection of AFS9800 spectrometer was 0.0989 μg/L.The method has the advantages of high sensitivity,high accuracy,high precision,wide linear range,simple operation and low reagent toxicity and,therefore,it can be widely used for determination of selenium in food and water.更多还原