【摘要】 目的:建立测定水产品中氯霉素残留量的ELISA分析法。方法:对不同样品采用不同的前处理方法,用ELISA方法进行检测。结果:方法检测限为0.01μg/kg;在0.5μg/kg和2.0μg/kg两个添加水平,样品加标平均回收率分别为72%～116%和86%～108%;重复性实验:RSD分别为1.8%～6.2%和6.5%～7.8%;重现性实验RSD分别为3.8%和5.9%。结论:本方法灵敏度、准确度和精密度均符合残留分析质量控制的要求,适用于水产品中氯霉素残留量的快速检测。更多还原

【Abstract】 Objective:To develop an enzyme immunoassay method for the detection of chloramphenicol(CAP) residues in aquatic products.Methods:CAP residues in different samples were extracted with different methods and detected an enzyme immunoassay kit detected.Results:The limit of detection(LOD) was 0.01 μg/kg.On the spiked levels of 0.5 μg/kg and 2.0 μg/kg,the mean recovery of ths method were 72%～116% and 86%～108%,respectively,with the RSD foe the repeatability of 1.8%～6.2% and 6.5%～7.8%,and the RSD for the reproducibility of 3.8% and 5.9%.Conclusion:The sensitivity,accuracy and precision of the method are consistent with the guide for quality control of residues analysis.It is suitable for the rapid detection of CAP residues in aquatic products.更多还原