【摘要】 目的:用PCR产物直接测序法和反向斑点杂交方法检测肺炎支原体(Mycoplasma pneumoniae,MP)23S rRNA V区2063基因,结果进行比较分析。方法:19例自临床咽拭子标本分离培养的MP,用自行设计的反向斑点杂交法检测23S rRNA V区2063基因型,同时对相应序列测序分析。结果:19例分离培养的MP,经反向斑点杂交法检测15株有23S rRNA V区基因A2063G位点突变;4株为2063A,与测序结果一致(Kappa一致性检验,P>0.05)。结论:反向斑点杂交方法能快速、准确检测临床MP 23S rRNA V区2063基因型。更多还原

【Abstract】 Objective:To establish a reverse dot-blot hybridization in detecting the genotypes of 23S rRNA domain V of Mycoplasma pneumoniae and to compare it with the direct sequencing method.Methods:A self-designed reverse dot-blot hybridization was applied to detect the genotypes of 23S rRNA domain V of Mycoplasma pneumoniae in 19 clinical isolates from oropharyngeal swabs,and the corresponding sequence was sequenced meanwhile.Results:15 strains of the 19 clinical isolates were found to possess point mutations of A2063G within domain V of the 23S rRNA gene by the reverse dot-blot hybridization,the other 4 strains were 2063A in domain V of the 23S rRNA gene.These findings were concordant with sequencing data(Kappa consistency test,P>0.05).Conclusion:The reverse dot-blot hybridization is capable of detecting the A2063G point mutation in domain V of 23S rRNA of Mycoplasma pneumoniae from isolates and patient specimens.更多还原