【摘要】 目的:探讨乙型肝炎患者病毒核酸载量与血清乙型肝炎病毒外膜大蛋白(HBV-LP)含量的关系。方法:采用酶联免疫吸附试验(ELISA)和实时荧光定量PCR法分别检测307例乙型肝炎患者血清的HBV-LP、HBV-M和HBVDNA,并进行相关性分析。结果:307例乙型肝炎患者血清中HBV-LP含量与HBV DNA拷贝数变化相一致,两者呈正相关(r=0.943);在131份HBeAg阳性血清中,HBV DNA与HBV-LP的阳性率分别为95.42%和93.89%,两者差异无显著性(P>0.05);在176份HBeAg阴性血清中,HBV DNA与HBV-LP的阳性率分别为46.02%和56.25%,两者差异无显著性(P>0.05)。结论:血清HBV-LP水平能反映HBV感染者体内HBV复制程度,其灵敏度高于HBeAg,可作为判断HBV复制新的血清学指标。更多还原

【Abstract】 Objective:To explore the correlation between the HBV DNA load and the content of HBV large envelope protein(HBV-LP) in chronic hepatitis B patients.Methods:Serum HBV DNA was quantitively detected by real-time polymerase chain reaction(RT-PCR),and the HBV-LP and HBV-M were detected by time differentiate immunofluorescence assay in 307 serum samples collected from chronic hepatitis B patients.Results:Serum HBV-LP level was closely correlated with HBV DNA copies(r=0.943) in 307 cases.There was no significant difference between positive rate of HBV-LP and that of HBV DNA(P>0.05) in 131 HBeAg-positive cases,the rates were 95.42% and 93.89%,respectively.The significant difference was neither found between positive rate of HBV-LP and that of HBV DNA(P>0.05) in 176 HBeAg-negative cases,the rates were 46.02% and 56.25%,respectively.Conclusion:The level of serum HBV-LP can be used to reflect the state of HBV replication and it is better than HBeAg in sensitivity.So it may be used as a new serological marker to reflect the HBV replication.更多还原