【摘要】 目的:了解东莞地区2006~2008年副溶血性弧菌(Vibrio parahaemolyticus,Vp)分离菌株毒力因子的变化情况。方法:用PCR法及神奈川实验检测毒力因子。结果:食物中毒分离菌株tdh、trh基因检出率分别为85.2%(127/149)、8.7%(13/149),外环境分离菌株tdh、trh基因检出率分别为6.3%(3/48)、2.1%(1/48),所有菌株神奈川实验阳性率达91.9%(137/149)。结论:东莞地区副溶血性弧菌致病力较强,致病菌株大多携带tdh基因,对市民身体健康的潜在危害较大,应进一步加强对副溶血性弧菌的监测研究。更多还原

【Abstract】 Objective:To investigate the virulence gene change of Vibrio parahaemolyticus from 2006 to 2008 in Dongguan.Methods:PCR method and Kanagaw phenomenon method was used to detect the virulence gene.Results:The positive rate of tdh and trh gene in the Vibrio parahaemolyticus strains from food poisoning samples was 85.2%(127/149) and 8.7%(13/149),respectively.And it was 6.3%(3/48) and 2.1%(1/48) from the environment samples.The positive rate of Kanagaw phenomenon for all the Vibrio parahaemolyticus strains was 91.9%(137/149).Conclusion:The pathogenicity of Vibrio parahaemolyticus strains in Dongguan is very strong,and most of them from food poisoning include tdh gene.It is necessary to strengthen the surveillance of Vibrio parahaemolyticus.更多还原