【摘要】 目的:研究食品原料蛤蟆油经60Co-γ射线照射后营养成分的变化。方法:采用0、5、10、15 kGyγ射线对蛤蟆油进行辐照,以加速试验来评价辐照前后营养成分变化与辐照剂量的相关性。结果:在5~15 kGy剂量范围内,5 kGy辐照前后维生素D,E含量变化均无显著性差异(P>0.05),而10~15 kGy辐照以及加速试验均使蛤蟆油中维生素A,E含量显著降低(P<0.01),维生素D受照射影响较小;脂肪酸在5~15 kGy辐照后变化不明显,但加速试验发现15 kGy使脂肪酸含量显著提高;经SDS-PAGE分析其分子量为20 KD的蛋白区带随辐照剂量增加而变深。结论:60Co照射动物性高蛋白、低脂肪、低维生素固体样品,在高辐照剂量下加速试验均有不同程度影响,但5 kGy剂量辐照还是安全的。更多还原

【Abstract】 Objective:To study the nutrient components changes in food material oviductus rana which is irradiated by 60Co-γ-ray.Methods:Oviductus rana was irradiated by 0,5,10,15 kGy γ-ray.The relationship between nutrient components changes and radiation dose was evaluated by accelerated test.Results:When the irradiation dose was between 5 and 15 kGy,the content of vitamin D and E had no significant difference(P>0.05).However,10 to 15 kGy irradiation and accelerated test made the content of vitamin A and E significantly lower(P<0.01).Vitamin D was less affected by irradiation.Total fatty acids content didn′t change significantly after 5 to 15 kGy irradiation.Accelerated test found that 15 kGy irradiation made fatty acids content significantly increased.Molecular weight 20 KD protein band tend darker as the irradiation dose added in SDS-PAGE analysis.Conclusion:60Co-γ-ray irradiation at high doses have effects on solid samples in different degree,which are of high animal protein,low fat and low vitamin.The 5 kGy dose of irradiation is safety.更多还原