【摘要】 目的:比较不同培养基对医院空气微生物检测结果的影响,探索一种简便实用的检测方法。方法:采用普通营养琼脂平板和血琼脂平板对医院空气微生物进行检测,并对结果进行比较分析。结果:普通营养琼脂平板检测细菌总数超标率为15.32%,均未检出金黄色葡萄球菌、溶血性链球菌;血琼脂平板检测细菌总数超标率为13.25%,检出金黄色葡萄球菌3份,溶血性链球菌1份。细菌总数超标率两者间差异无显著性(2χ=0.68,P>0.05),致病菌检测两者间差异有显著性(2χ=4.02,P<0.05);致病菌检测后者优于前者。两种平板上的平均菌落数在0~1 cfu/平板至>7 cfu/平板各区间分布的检测数差异均无显著性(P>0.05)。结论:用血琼脂平板对医院空气检测结果优于普通营养琼脂平板,尤其是致病菌检测。更多还原

【Abstract】 Objective:To explore a simple and practical detection method by comparing the microbiological test results in air in the hospital.Methods:One culture medium with ordinary nutrient agar plate and the other with blood agar plate were used to detect the microorganisms in the air in the hospital,and the results were used to be a comparative analysis.Results:By using the culture medium with ordinary nutrient agar plate,the overall overproof rate of total amount of bacteria was 15.32%,and Staphylcoccus aureus and Hemolysis streptococcus were not found.By using blood agar plate,the overproof rate was 13.25%,and three strains of Staphylcoccus aureus and one strain of Hemolysis streptococcus were found.The overproof of amount of bacteria between the two culture mediums have no statistical significance(χ2=0.68,P>0.05),while the ones of pathogenic bacteria have statistical significance(χ2=4.02,P<0.05).The inspection of pathogenic bacteria of the latter culture medium was better than the former.There was no statistical significant diffence in the average number of the bacteria(from 0~1 cfu/plate to >7 cfu/plate) on the both culture mediums(P>0.05).Conclusion:The way of using the culture medium with blood agar to detect the microorganisms in the air in in the hospital is better than ordinary nutrient agar plate,especially for detecting the pathogenic bacteria.更多还原