【摘要】 目的分析AKT1基因PH(the pleckstrin homology,PH)结构域第4外显子的碱基序列,探讨中国人和日本人AKT1基因突变与胃肠道肿瘤的关系。方法采用激光显微切割技术捕获胃癌组织和大肠癌组织的肿瘤细胞,以这些标本的基因组DNA为模板,用聚合酶链反应PCR方法扩增AKT1基因PH结构域,PCR产物经纯化后直接测序。结果分析测序图发现AKT1基因PH结构域第4外显子第49核苷酸没有出现点突变。结论中国人和日本人的胃肠道肿瘤的AKT1基因PH结构域没有出现点突变,与胃肠道肿瘤发生发展关系不大。更多还原

【Abstract】 Objective To Analysis of AKT1 gene PH(the pleckstrin homology,PH) domain in exon 4 of the base sequence,study the relations of Chinese and Japanese AKT1 gene mutation and gastrointestinal tumor.Methods The tumor cells were got from Gastric cancer and colorectal cancer tissue by Laser microdissection.The the PH domain of AKT1 gene were Amplificated by RT-PCR.PCR products were purified and directly sequenced.Results PH domain of AKT1 gene in exon 4 of 49 nucleotide mutation does not appear Point mutation.Conclusion PH domain of AKT1 gene of Chinese and Japanese gastrointestinal tumor does not appear Point mutation,the development of gastrointestinal cancer has little to do with it.更多还原