【摘要】 目的探讨Egr-内皮抑素基因联合放射性药物-胶体32P对于大鼠肝癌的抑制效果。方法经局部引入基因重组质粒及胶体32P后于,7 d1、4 d2、1 d分别测肝癌的生长率,第21d测定肝癌微血管密度(MVD)、肿瘤细胞凋亡指数(AI)等指标的变化。结果各干预组的肿瘤体积生长率、MVD、AI与对照组指标相比均具有统计学差异,P均<0.05-0.01,在所有干预组中,32P+Egr-Endostatin组的抑瘤效果最好,P均<0.05-0.01。肝癌生长率方面,第7 d-14d,32P干预组与单纯内皮抑素基因组、Egr-内皮抑素基因组相比抑瘤效果相近,P>0.05;但到达21 d时,胶体32P干预组治疗效果则明显下降。基因联合胶体32P的干预组效果均好于其它干预组,P<0.05-0.01。MVD方面,32P组与其它干预组比较具有统计学差异,P<0.01。AI指标,其他干预组与32P组比较具有差异,P<0.05。结论 32P联合Egr-Endostatin基因协同抑瘤,可以发挥放射性药物的照射作用,Eg-1的辐射诱导增强作用及Endostatin基因的抑制肿瘤新生血管的三重作用,疗效最为确切。更多还原

【Abstract】 Objective To investigate the antitumor effect of EgrEndostatin gene combined with radioactive drug-colloidal 32P inhibitory on the rat liver.Methods After gene and colloidal 32 P were injected into liver cancer,the growth rate of liver cancer were measured at 7 d,14 d,21 d.Microvessel density determination(MVD)and apoptosis index(AI) were be measured at 21 d.Results Compared with the control the indexs in the treatment groups had significant difference index(P<0.05-0.01).The inhibitory effect of 32 P+ Egr-Endostatin is the best(P<0.01).In the term of liver cancer growth rate,there are not significant difference between the 32 P group,Endostatin gene and Egr-Endostatin gene group in antitumor effect at 7 d-14 d,(P>0.05);But at 21 d,the treatment effect of 32 P group is clearly down.In terms of MVD,compared with the control group the treatment group has obvious statistical difference(P<0.01);compared with the other treatment group,32P group has statistical difference(P<0.01);32 P+Endostatin group has statistical comparing with other groups(P<0.01).In terms of AI index,the control group has statistical difference comparing with treatment groups(P<0.01);32P group also has difference comparing with Endostatin group and Egr-Endostatin group(P<0.05);AI index of 32 P+Endostatin group and 32 P+Egr-Endostatin is better than other treatment groups(P<0.05-0.01).Conclusion Colloidal 32 P combining Egr-Endostatin gene plays the important role in treating liver cancer.32P can create the radiation function,Egr-1 can induce expression enhancement of Endostatin and the Endostatin Gene can inhibit liver tumor angiogenesis.Therefore the whole effect of the three factors can manifestly decrease the liver cancer growing.更多还原