节点文献

多西环素人工抗原的合成与鉴定

Synthesis and Identification of Antigens of Doxycycline

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 乐涛严佩峰王喜亮郭延成沈亚安毕丁仁

【Author】 LE Tao1,2,YAN Pei-feng2,WANG Xi-liang1,GUO Yan-cheng1,SHEN Ya-an1,BI Ding-ren1(1.State Key Laboratory of Agricultural Microbiology,College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,PR China;2.Xinyang Agricultural College,Xinyang,Henan 464000,P.R.China)

【机构】 华中农业大学动物医学院农业微生物学国家重点实验室信阳农业高等专科学校动物科学系

【摘要】 将多西环素(doxycycline,DOX)进行化学修饰引入羧基或氨基等活性基团,然后与牛血清白蛋白(BSA)和卵清蛋白(OVA)偶联合成人工免疫原DOX-PABA-BSA、DOX-BSA和包被原DOX-PABA-OVA、DOX-OVA,并用紫外吸收(UV)、凝胶电泳(SDS-PAGE)和ELISA方法对人工抗原进行鉴定;将合成的人工抗原DOX-PABA-BSA、DOX-BSA分别免疫BALB/C小鼠,用间接ELISA方法测定多抗(pAb)效价,用竞争ELISA方法鉴定其敏感性,用交叉反应试验鉴定其特异性。结果表明,二个免疫组6只小鼠血清抗体效价均在1∶6400以上,DOXpAb对DOX的50%抑制浓度(IC50)在39.79~53.13μg/L,抗血清与四环素类药物交叉反应很低。本实验为建立多西环素ELISA残留免疫学检测方法和并制备多西环素试剂盒奠定了基础。

【Abstract】 Doxycycline(DOX)was modified with carboxyl or amino-active group,and then with bovine serum albumin(BSA)and ovalbumin(OVA)which were coupled synthesis of artificial immunogen DOX-PABA-BSA,DOX-BSA and coated antigen DOX-PABA-OVA,DOX-OVA.UV Absorption(UV),sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) and ELISA were identified on the artificial antigen.The synthesis of artificial antigen DOX-PABA-BSA,DOX-BSA were immunized BALB/C mice.Polyclonal antibody titer were measured by indirect ELISA,using competitive ELISA sensitivity was identified,using cross-reaction tests identify their specificity.The results showed that two immunization groups of six mice with anti-serum titer higher than 1∶6400 by indirect ELISA,50% inhibitive concentration(IC50) was 39.79~53.13 μg/L and low levels of cross-reactivity to Tetracyclines were observed.In this study,In this study,the Pab(anti-DOX antiserum) can be used to develop a ELISA kit to detect DOX residues in animal edible tissues.

【关键词】 多西环素半抗原完全抗原合成鉴定
【Key words】 doxycyclinehaptencomplete antigensynthesisidentification
【基金】 国家科技支撑项目(2006BAK02A09)
  • 【文献出处】 中国兽药杂志 ,Chinese Journal of Veterinary Drug , 编辑部邮箱 ,2010年01期
  • 【分类号】S859.84
  • 【被引频次】7
  • 【下载频次】262
节点文献中: 

本文链接的文献网络图示:

本文的引文网络