【摘要】 目的建立弓形杆菌的分离培养方法。方法通过纤维滤膜过滤的方法对菌悬液过滤,用CCDA平板对弓形杆菌进行分离培养。将分离到的可疑菌落进行分纯培养,然后通过生化和16SrRNA序列分析进行鉴定。结果从鱼肠内容物中分离到一株疑似菌株,对其进行表型生化分析和16SrDNA全序列分析,鉴定该菌为布氏弓形杆菌。结论该检验方法适用于对弓形杆菌进行分离培养。更多还原

【Abstract】 Objective To explore a method for screening Arcobacter spp. Method Filtering the suspensions containing Arcobacter with a fiber filter membrane technique,and using CCDA plates to isolate the strain. Results One suspected strain was isolated from fish. The phenotype characters and 16S rDNA of this strain were analyzed and identified as Arcobacter butzleri. Conclusion The method is reliable for screening Arcobacter spp.更多还原