【摘要】 目的:观察真武汤对肾间质纤维化大鼠的作用,并通过观察真武汤对肾脏Ⅰ,Ⅲ型胶原纤维的影响,探讨真武汤抗肾间质纤维化的作用机制。方法:采用"单肾切除加腺嘌呤灌胃法"建立大鼠慢性肾衰竭模型。治疗组予真武汤9.8,2.45g.kg-1 ig,对照组予尿毒清溶液ig。给药4周后处死大鼠取血测肌酐(Cr),尿素氮(BUN),钙(Ca2+)、磷(P3+),肾脏切片分别进行HE染色、PASM染色、Masson染色、Sirius Red染色、Mallory染色,光镜下观察大鼠肾组织的病理形态学改变,HE染色进行肾小管间质病变半定量分析,PASM染色、Masson染色、Sirius Red染色进行肾脏组织病理学图像分析。结果:在肾间质纤维化面积、肾小球系膜积分吸光度等方面,真武汤高、低剂量组的作用优于模型组和尿毒清组,P<0.05。真武汤高、低剂量组在肾间质中Ⅰ,Ⅲ型胶原分布面积等方面的作用与模型组比,P<0.05。结论:真武汤具有抗肾间质纤维化的作用,其作用途径可能与抑制Ⅰ,Ⅲ型胶原纤维增生有关。更多还原

【Abstract】 Objective:Observing the protective effect of Zhenwu Decoction on renal interstitial fibrosis in rat,to explore its role of anti-renal fibrosis and Ⅰ,Ⅲ collagen fibers affected in renal fibrosis.Method:The rat model of chronic renal failure was used.Niaoduqing control group was treated by Niaoduqing solution,Zhenwu Decoction group was treated by Zhenwu Decoction concentrated solution,up to 28 days.After 4 weeks,blood samples were taken from the femoral artery.Serum creatinine,blood urea nitrogen,serum calcium and phosphorus were detected.Renal pathological staining for paraffin sections was carried out by hemotoxylin and eosin staining,periodic acid-silver methenamine(PASM) staining,Masson staining,Sirius red staining,Mallory staining.HE staining was randomly selected for semi-quantitative analysis in tubulointerstitial lesions.Periodic acid-silver methenamine staining,Masson staining,Sirius red staining,Mallory staining were randomly selected for kidney pathology analysis.Result:In PASM mesangial integral optical density quantitative model of mesangial proliferative,Masson staining fibrosis in the area of renal interstitial,Low-dosage Zhenwu Decoction and High-dosage ZhenwuDecoction group appeared to be better than model group and Niaoduqing control group(P < 0.05).Low-dosage Zhenwu Decoction and High-dosage Zhenwu Decoction group appeared to be less than model group in renal interstitial of type Ⅰ and Ⅲ distribution area(P < 0.05).Conclusion:Zhenwu Decoction shows effect of anti-renal interstitial fibrosis.And it inhibit Ⅰ,Ⅱ collagen fibers proliferation,which may be the mechanism for Zhenwu Decoction to treat renal interstitial fibrosis and chronic renal failure.更多还原