【摘要】 目的:筛选一种方法简便、稳定、成石效果好的实验性大鼠肾草酸钙结石造模方法。方法:采用几种目前常用的实验性大鼠肾草酸钙结石造模方法(造模Ⅰ组:1%乙二醇饮水+2%氯化铵每天ig 2 mL/只;造模Ⅱ组:1.25%乙二醇+1%氯化铵,ig 20 mL.kg-1.d-1;造模Ⅲ组:每只每天饮用1.25%乙二醇+1%氯化铵10 mL,水30 mL;造模Ⅳ组:1%乙二醇+1%氯化铵自由饮用)进行造模,并进行各组尿液、血液和肾组织中的多项生化指标【尿素氮(BUN)、肌酐(Cr)、尿酸(UA)、无机磷(IP)、钙(Ca2+)、草酸(OX)】检测以及肾脏病理标本观察,综合评价造模效果。结果:造模Ⅰ组的24 h尿液中的BUN,Cr,UA,IP,Ca2+,OX均高于正常对照组(Ⅴ组),且统计学上有极显著性差异(P<0.01);血液中BUN,Cr,UA,IP,Ca2+均高于正常对照组,且统计学上有极显著性差异(P<0.01);肾组织中Ca2+和OX均高于正常对照组,且统计学上有极显著性差异(P<0.01);肾组织可见大量草酸钙结晶沉积。造模Ⅱ组和造模Ⅲ组的多项生化指标结果显示大鼠的肾功能下降,但肾组织中未见结晶体,仅发现肾间质血管充血。造模Ⅳ组大鼠全部死亡。结论:造模Ⅰ组为最佳造模方法。更多还原

【Abstract】 Objective:To select a simple,stable and satisfied experimental model of renal calcium oxalate calculus in rats.Method:After several renal calcium oxalate calculus models currently used(Model group I:administered intragastrically with 1% ethylene glycol free drink + 2% ammonium chloride 2 mL/rat per day;model Group Ⅱ:administered intragastrically with 1.25% ethylene glycol + 1% ammonium chloride 20 mL.kg-1 per day;model group Ⅲ:drank 10 mL 1.25% ethylene glycol + 1% ammonium chloride and 30 mL water per day;model group Ⅳ:drank 1% ethylene glycol + 1% ammonium chloride freely.) experimental rat models were developed.Several biochemical indicators[urea nitrogen(BUN),creatinine(Cr),uric acid(UA),inorganic phosphorus(IP),calcium(Ca2 +),oxalate(OX)] of urine,blood and nephridial tissue were measured and renal pathological specimens were observed.Then the modeling results were comprehensive evaluated.Result:The 24 h urinary BUN,Cr,UA,IP,Ca2 +,OX of model groupⅠwere singnificantly higher than the normal control group(groupⅤ)(P < 0.01);blood BUN,Cr,UA,IP,Ca2 + were significantly higher than the normal control group(P < 0.01);nephridial tissue Ca2 + and OX were significantly higher than the normal control group(P < 0.01);and calcium oxalate crystals were distributed in nephridial tissue.The several biochemical indicators of model group Ⅱ and Model group Ⅲ disclosed the rats’ renal function breakdown,and renal pathological specimens only displayed vascular engorgement in renal interstitium and calcium oxalate crystals hadn’t been seen in nephridial tissue.All rats of model group Ⅳ were dead.Conclusion:Model groupⅠrepresents the best modeling for renal calculus of calcium oxalate.更多还原