【摘要】 目的监测福建省甲型H1N1流感病毒NA基因的变化以及对达菲的耐药情况,为临床诊疗和疾病控制提供参考依据。方法从福建省流感监测网络中随机选取23株甲型H1N1流感病毒,经病毒核酸提取和一步法RT-PCR扩增,获得NA基因片段,双向测定核苷酸序列,分析NA基因序列和重要氨基酸位点特征。结果 23株甲型H1N1流感病毒NA片段基因与A/California/07/2009(H1N1)代表株的核苷酸序列进行比较,同源性高达98.1%以上;23株毒株NA蛋白第275位氨基酸均为组氨酸。结论随机选取的23株甲型H1N1流感病毒NA基因片段保持高度同源并且对达菲仍然敏感。随着国内外达菲耐药株的不断出现,应加强耐药性监测,为制定应对甲型H1N1流感流行措施提供参考。更多还原

【Abstract】 To investigate characteristics of NA genes and oseltamivir susceptibility of 2009 pandemic H1N1 influenza viruses in Fujian Province,twenty-three strains were randomly selected for detection.Fragments of NA genes were amplified by one-step RT-PCR and then were sequenced.Comparing with the A/California/07/2009(H1N1),alignment of nucleic acid sequences revealed a high homology of 98.1%.While the amino acid residuals at 275 remained histidine,indicating all of the 23 isolates were oseltamivir-sensitive.This observation suggests no oseltamivir-resistant strains has emerged in Fujian.However,continuous surveillance is necessary for control and prevention strategies of the ongoing pandemic.更多还原