【摘要】 目的比较研究小鼠经不同途径接种日本血吸虫(Schistosoma japonicum,Sj)童虫原代细胞(primary juvenile worm cells,pJCs)后所诱导的免疫保护效果,寻找其合适的免疫途径。方法将pJCs经皮下或静脉注射途径免疫昆明小鼠,间隔2w共免疫3次,末次免疫后第4w,与对照组同时经腹部皮肤感染尾蚴30±2尾/鼠,比较三组小鼠的肝脏虫卵数及虫卵肉芽肿的大小、成虫数、虫体大小。同时在第2、3次免疫前及攻击感染前1d,小鼠尾静脉采血,用ELISA法检测抗pJCs-IgG水平。结果 pJCs免疫小鼠后,与PBS对照组比,静脉免疫组的减虫率为48.53%、肝卵减少率为54.54%、虫体平均重量减少率为29.62%(P<0.01)、虫卵肉芽肿面积减少率为36.8%,而皮下免疫组分别为41.28%、43.19%、23.08%、31.2%。IgG抗体水平也是静脉注射组高(PBS组,P<0.01;皮下注射组,P<0.05)。结论日本血吸虫童虫细胞免疫原通过皮下和静脉注射均可诱导小鼠产生对日本血吸虫的免疫保护力,其中静脉注射诱导的免疫保护效应最强,提示通过静脉注射日本血吸虫童虫细胞是可行有效的免疫途径。更多还原

【Abstract】 In order to observe the influence of immunization route on the immune protective effects induced by primary juvenile worm cells(pJCs),Kunming mice were inoculated intravenously or subcutaneously with pJCs from 12 day-old Schistosoma japonicum respectively.All mice were immunized three times at 2 weeks interval and were challenged each with 30±2 cercariae by abdominal skin penetration 4 weeks after the last booster.Six weeks of post-infection,mice were sacrificed by aether inhalation,and the numbers of worms and hepatic eggs were counted.Specific anti-pJCs levels in the mice sera collected one day before the second,third immunization and challenge infection were tested with ELISA.Mice immunized with pJCs given i.v.or s.c.exhibited high level of specific antibody and protection effects,but given i.v.was significantly higher than given s.c.in anti-pJCs level(P<0.05)and showed significant reduction in worm burden(48.53%),liver eggs per gram(LEPG)load(54.54%),worm average weight(29.62%)and egg granuloma size(36.8%).The juvenile worm cells of S.japonicum could induce partial protective immunity against S.japonicum in mice and the protective immunity by i.v.was better than that by s.c..These results indicated intravenous route was a feasible and efficacious route for juvenile worm cell immunization.更多还原