【摘要】 目的对西城区2005-2007年来收集的志贺菌进行毒力相关基因检测并分析其分布情况,同时建立多重PCR方法检测志贺菌。方法利用志贺菌中四个主要毒力相关基因ipaH、ial、set1A、set1B的引物分别对所收集的志贺菌进行PCR方法检测与分析,并且建立了毒力相关基因多重PCR方法的反应条件。结果ipaH基因存在于所有志贺菌中,set1A、set1B基因仅存在福氏志贺菌中,ial基因在反复复苏的菌株中检出率有所下降,尤其以宋内缺失率高(49%);多重PCR方法检测志贺菌方便快捷,而且可以初步分型。结论志贺菌菌型分布发生了变化,毒力相关基因的分布也相应地有所不同;多重PCR具有志贺菌的快速诊断价值。更多还原

【Abstract】 The objective of the present study was to detect the virulence-associated genes of Shigella species in Xicheng district of Beijing and in order to develop the multiplex PCR method for diagnosis.From 2005 to 2007,a total of 95 strains were detected and four sets of primers were applied to amplify ipaH,ial,set1A and set1B genes,respectively.At the same time,a reaction condition was established for the multiplex PCR.Results indicated that the ipaH gene was present in all Shigella strains but the set1A and set1B genes were only present in all Shigella flexneri strains.Moreover,the positive rate of the ial gene in repeated recovery Shigella strains was decreased,and the deletion rate of Shigella sonnei was relatively higher(49%).Results indicated that the diverse distributions of the virulence-associated genes were according to the various distributions of strain types of Shigella.The multiplex PCR method developed a quicker and more convenient way for Shigella strains typing,and it had clinic significance for diagnosis in Shigella species.更多还原