【摘要】 为了明确西瓜种质资源耐低温性,为耐低温育种工作提供理论依据,对21份西瓜种质资源在人工模拟气候条件下进行冷害指数、电导率、可溶性蛋白含量、超氧化物歧化酶(SOD)、过氧化物酶(POD)活性和丙二醛(MDA)含量的测定,并对基于被测的各项指标的结果进行了聚类分析。不同品种耐冷指数不同,其中野生西瓜材料耐冷指数最小,ZXG01315最大;供试品种中电导伤害率存在一定差异,野生西瓜的电导伤害率最小,超甜早霸母本电导伤害率最大;随低温胁迫叶绿素a/b值的变化分为降低、上升和平稳三种类型;可溶性蛋白和MDA含量随温度降低而增加;SOD和POD活性随温度降低而升高;聚类分析表明,西瓜材料分为三大类,其中类群I只包含野生西瓜;类群II、III各包含10份材料。类群与西瓜耐冷性存在一定的相关性。第一和第三类群可作为耐低温育种的育种材料。更多还原

【Abstract】 The objective of this experiment was to analyze watermelon germplasm for resistance to low temperature and provide theoretical basis for breeding. 21 watermelon materials were tested by chilling index, electric conductivity, soluble protein, SOD, POD, and MDA in man-made simulate climate and all the results were clustered. Differences were found in chilling index among watermelon varieties. Wild watermelon chilling enduring level was higher than that of others while ZXG01315 was the lowest. There are significant differences in the electric conductivity. Wild watermelon was the lowest while super-sweet female parent was higher than that of others. The change of chlorophyll a/b in the lower temperature stress was divided into three types which were lower, rise and smooth. The more chilling enduring higher; the more soluble protein content and malondialdehyde increased greater. The superoxide dismutase activity and peroxidase activity increased along with the temperature cut down. The cluster analysis divided the 21 varieties to 3 parts: the first part included only wild watermelon; the second and the third parts both included 10 watermelon materials. Watermelon parts have a close relationship with watermelon chilling enduring. The first and the third parts can be used as breeding materials.更多还原