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微波法提取新疆罗布麻叶总黄酮及其抗氧化能力的研究
Microwave extraction technique and anti-oxidation activity of total flavonoid from Xinjiang apocynum venetum leaves
【摘要】 采用L(934)正交试验对新疆罗布麻叶的微波提取工艺进行优选,以黄酮提取量为参考指标,确定最佳提取工艺条件为:1:12(g/mL)的80%乙醇水溶液,70℃微波提取10min,提取4次,罗布麻叶提取总黄酮含量为56mg/g。研究了提取物的抗氧化能力,新疆罗布麻叶总黄酮对DPPH自由基有强的清除活性,IC50值为6.7ug/mL,抗氧化效果优于芦丁次于VC。
【Abstract】 Experiments were carried out by using L9 (34) orthogonal design with yield of total flavonoids as the index.The optimal extraction process was determined as follows:the ratio of 80% C2H5OH and water was 1:12 (g/ml),temperature was 70℃,time was 10min,microwave extraction for four times.The yield of total flavonoids from apocynum venetum leaves was 56mg/g.The anti-oxidation activity of flavonoid was studied as well:the flavonoids from Xinjiang apocynum venetum leaves had powerful scavenging efficiency to DPPH free radical.Its IC50 was 6.7ug/mL.The effect of anti-oxidation was better than rutin but less than VC.
【Key words】 microwave extraction; apocynum venetum leaves; totaI flavonoids; DPPH;
- 【文献出处】 中国酿造 ,China Brewing , 编辑部邮箱 ,2010年01期
- 【分类号】TQ461
- 【被引频次】7
- 【下载频次】337