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Production and characterization of biosurfactant from Bacillus subtilis CCTCC AB93108

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【作者】 刘智峰曾光明钟华傅海燕刘小兰

【Author】 LIU Zhi-feng1, 2, ZENG Guang-ming1, 2, ZHONG Hua1, 2, FU Hai-yan1, 3, LIU Xiao-lan1 1. College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; 2. Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan University, Changsha 410082, China; 3. Department of Environmental Engineering, Xiamen University of Technology, Xiamen 361024, China

【机构】 College of Environmental Science and Engineering, Hunan UniversityKey Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan UniversityDepartment of Environmental Engineering, Xiamen University of Technology

【摘要】 The production and properties of the biosurfactant synthesized by Bacillus subtilis CCTCC AB93108 were studied. The maximum concentration of the surfactant is 1.64 g/L when the bacteria grow in a medium supplemented with glucose as carbon sources. The isolated biosurfactant is a complex of protein and polysaccharide without lipids. It reduces the surface tension of distilled water to 45.9 mN/m, and its critical micelle concentration (CMC) is 2.96 g/L. It can stabilize emulsions of several aromatic and aliphatic hydrocarbons, such as benzene, xylene, n-pentane, n-nonane, gasoline and diesel oil. It presents high emulsification activity and stability in a wide range of temperature (4-100 ℃) and a long period of duration.

【Abstract】 The production and properties of the biosurfactant synthesized by Bacillus subtilis CCTCC AB93108 were studied. The maximum concentration of the surfactant is 1.64 g/L when the bacteria grow in a medium supplemented with glucose as carbon sources. The isolated biosurfactant is a complex of protein and polysaccharide without lipids. It reduces the surface tension of distilled water to 45.9 mN/m, and its critical micelle concentration (CMC) is 2.96 g/L. It can stabilize emulsions of several aromatic and aliphatic hydrocarbons, such as benzene, xylene, n-pentane, n-nonane, gasoline and diesel oil. It presents high emulsification activity and stability in a wide range of temperature (4-100 ℃) and a long period of duration.

【基金】 Project(IRT0719) supported by the Program for Changjiang Scholars and Innovative Research Team in University of China; Project (2004AA649370) supported by the National High-Tech Research and Development Program of China; Projects(50425927, 50225926) supported by the Natural Foundation for Distinguished Young Scholars; Project(2007F3102) supported by Technology Foundation for Creative Young Scholars of Fujian Province, China
  • 【文献出处】 Journal of Central South University of Technology ,中南大学学报(英文版) , 编辑部邮箱 ,2010年03期
  • 【分类号】TQ423
  • 【被引频次】1
  • 【下载频次】58
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