【摘要】 目的:探讨99mTc标记抗血小板板膜单克隆抗体SZ-21在兔股动脉血栓模型放免显像中的应用价值。方法:将抗体用2-IT法修饰后进行99Tcm标记;5只血栓模型兔耳缘静脉注射99Tcm-SZ-21后进行SPECT平面显像,观察血栓部位放射性分布并勾划感兴趣区(ROI),计算血栓与周围肌肉(T/M)和血栓与对侧血管(T/B)ROI比值。1只模型兔注射99Tcm-GH,作为阴性对照。结果:注射99Tcm-SZ-21后30分钟,血栓开始显影,至注射后2小时,血栓影像清晰;血栓模型活体显像时T/M和T/B比值分别为2.55±0.72和1.94±0.51,离体显像时T/B比值为4.43±1.5。结论:99Tcm-SZ-21与新鲜血栓具有较好的结合能力,靶与非靶组织比值高,能够对血栓进行早期、快速地成像。更多还原

【Abstract】 Objective:To label monoclonal anti-GPⅢa antibody (SZ21) with 99 Tcm and evaluate the value of 99 Tcm-SZ-21 for detecting thrombi in vivo.Methods:SZ-21 was modified with 2-iminotholane and labeled with 99 Tcm-GH and radiochemical purity was determined by ITLC-SG.99Tcm-SZ-21 was injected via ear edge vein in 5 rabbits in which thrombi were induced in the right femoral arteries.As control,99 Tcm-GH was administered in 1 rabbit.Static images were acquired and irregularly shaped ROIs were drawn on the images to calculate the ratios of T/M and T/B.Vein blood was drawn at 2 min,5 min,10 min,30 min and 1 h-3 h after injection in 2 rabbits so as to observe the blood clearance of 99 Tcm-SZ-21.Rabbits were sacrificed after 3 h of imaging.The vessels including clots were harvested and imaged.Cardiac muscle,liver,spleen,lung,kidney,etc.were excised and weighed.Radioactivity counts were measured to calculate % ID/g.Results:The radiochemical purity of 99 Tcm-SZ21 was beyond 90% and stable in vitro.Thrombi could be visualized at 30 min after injection,and at 2 h image of thrombi was clearly visualized,T/M (2.55±0.72) and T/B (1.94±0.51) ratios were high.In vitro imaging showed that T/B was 4.43±1.5.Conclusion:99Tcm-SZ-21 could be a potential agent for imaging diagnosis of thrombotic disease.更多还原