【摘要】 目的探讨人参皂甙(ginsenoside Rh2,G-Rh2)对动脉粥样硬化大鼠血脂、血清NO、MDA含量、SOD活力的影响.方法将45只Wistar大鼠随机分为对照组、模型组、Rh2组,每组15只.对照组大鼠喂饲普通饲料,模型组及Rh2组大鼠采用高脂饮食加维生素D3(总量7×105U/kg,分别于第1周和第6周腹腔注射)建立大鼠动脉粥样硬化模型.Rh2组大鼠高脂喂养同时1次/d Rh2200 mg/(kg.d)-1灌胃,每周称体质量1次,根据体质量变化调整给药量;对照组、模型组大鼠以生理盐水灌胃.11周后颈动脉采血,检测血脂水平,血清一氧化氮(NO)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平.结果 1)Rh2对血脂的影响:与对照组相比较,模型组大鼠血清TC,LDL-c及AI显著升高(P<0.01);与模型组比较,Rh2给药组TC,LDL-c和AI明显降低,差异显著(分别P<0.01,P<0.01,P<0.05);而高密度脂蛋白、血清三酰甘油水平相比无统计学意义.2)Rh2对血清NO含量的影响:与对照组相比较,模型组大鼠血清NO含量明显降低(P<0.01);与模型组比较,Rh2组NO含量明显升高(P<0.05);药物组与对照组相比无统计学意义.3)Rh2对血清MDA含量与SOD活力的影响:模型组MDA水平与对照组相比明显升高(P<0.01),而SOD活力明显降低(P<0.01).与模型组相比,Rh2对血清SOD活力有明显升高的作用(P<0.05);同时,Rh2具有显著降低MDA水平的作用(P<0.05).Rh2组与对照组相比无统计学意义.结论 Rh2通过降低高脂大鼠血清低密度脂蛋白和总胆固醇水平,提升高脂大鼠血清NO水平、血清SOD活力,降低MDA含量,增强抗脂质过氧化功能和清除氧自由基,稳定细胞膜,保护内皮细胞而发挥抗AS作用.更多还原

【Abstract】 Objective To investigate the effects of Rh2 on lipemia,NO,MDA and SOD in the atherosclerosal Rats.MethodExperimental atherosclerosis in rats was produced by feeding with high fat diet and administering vitamin D3(7×105 U/kg) in intraperitoneal injection at the first week and the sixth week respectively.Healthy forty-five male or female Wistar rats,whose body weights range from 180 g to 230 g.The three groups were normal group(feed on normal diet),AS model group(feed on high fat diet) and Rh2 group(feed on high fat diet and Rh2 200 mg/(kg·d).The former two groups were given isotonic sodium chloride instead of Rh2accordingly.The rats of each group were weighed once a week and the dosages were adjusted to the changes of body weight accordingly.After the rats were fed for successive 11 weeks,serum levels of total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),Nitric oxide(NO),Superoxide dismutase(SOD),Malondialdehyde(MDA) were detected respectively.Results1)The effect of Rh2 on blood lipoproteins:The serum level of TC,LDL-c and AI of Model group was significantly higher than that of Control group(P<0.01);Rh2 decreased the serum level of TC,LDL-c,and AI significantly.The difference of each index between Model group and Rh2 group was remarkable(P<0.01,P<0.01,P<0.05)but there was no statistic difference between them in TG and HDL-c.2)The effect of Rh2 on the serum NO level:The serum NO level of Rh2 group was higher than that of model group(P<0.05).The NO level of control group was higher than that of model group(P<0.01);The difference in NO level between Rh2 group and control group was not remarkable statistically.3)The effects of Rh2 on the serum MDA level and SOD activity:MDA level of model group increased obviously compared with that of control group(P<0.01).SOD activity of model group decreased obviously compared with that of control group(P<0.01).Rh2 highly improved the serum SOD activity compared with model group(P<0.05) and highly decreased the serum MDA level compared with model group(P<0.05).The differences in MDA level and SOD activity between Rh2 and control group were not remarkable statistically.ConclusionBy decreasing serum levels of TC,LDL-c,by improving serum levels of NO,by improving activity of SOD and decreasing the level of MDA,Rh2 would protect vascular endothelium and prevent AS in this way.更多还原