【摘要】 目的建立黄芡降糖片的准确定量质控方法.方法采用高效液相色谱法(HPLC),以YMC-C18(250 mm×4.6 mm,5μm)为色谱柱,以乙腈-水(48∶52)(含0.34%磷酸二氢钾和0.17%十二烷基硫酸钠)为流动相,流速为1.0 mL/min,检测波长为265 nm,柱温为25℃,测定黄芡降糖片中主要成分盐酸小檗碱的含量.结果盐酸小檗碱在0.64～6.40μg内线性关系良好,r=0.999 5,加样回收率为99.86%,RSD=1.01%.结论该方法操作简单、方便,重现性好,用于控制黄芡降糖片的含量,可信度高.更多还原

【Abstract】 Objective To establish an accurate method used for the control in quantity and quality of Huangqian(HQ).Method HPLC method was used for the determination of Berberine hydrochloride in HQ on YMC-C18(250 mm×4.6 mm,5 μm)chromatographic column;acetonitrile-water(48∶52)(including 0.34% potassium phosphate dibasic solution and 0.17% sodium dodecyl sulfate) was mobile phase;the wavelength was 265 nm;the flow rate was 1.0 mL/min and the column temperature was 25 ℃.Results The linear range of Berberine hydrochloride in HQ was 0.64～6.40 μg,r=0.999 5,the average recovery was 99.86%,RSD=1.01%.Conclusion A simple,accurate and excellent in reappearance method to Berberine hydrochloride in HQ was established.It is reliable to use it to control content of Berberine hydrochloride in HQ.更多还原