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siRNA沉默Annexin-1基因对膀胱癌T24细胞顺铂耐药的影响

The changes of DDP sensitivity after annexin-1 silencing by small interfering RNA of bladder cancer T24 cells

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【作者】 高琳杨远航徐万海林相国李建章杨德君王晓民

【Author】 GAO Lin,YANG Yuan-Hang,XU Wan-Hai,et al.Department of Urology,the Fourth Affiliated Hospital of Harbin Medical University,Harbin 150001,Heilongjiang,China

【机构】 哈尔滨医科大学附属第四医院泌尿外科澳大利亚Monash大学

【摘要】 目的观察RNA干扰沉默膜联蛋白-1(Annexin-1)基因表达技术对膀胱癌T24细胞Annexin-1基因表达和顺铂耐药的影响。方法针对Annexin-1基因不同部位设计并化学合成3对不同的靶向小分子干扰RNA(siRNA),脂质体介导瞬时转染膀胱癌T24细胞,转染后应用半定量RT-PCR和Western印迹检测Annexin-1mRNA和蛋白的改变,用MTT法检测沉默Annexin-1表达在膀胱癌T24细胞对顺铂敏感性的变化。结果转染siRNA后膀胱癌T24细胞Annexin-1的mRNA和蛋白水平显著下降(P<0.05),增强细胞对顺铂的敏感性(P<0.05)。结论 RNA干扰Annexin-1基因后其mRNA和蛋白水平显著下降,并且增强T24细胞对顺铂的敏感性。

【Abstract】 Objective To explore the effects of annexin-1 gene silencing by small interfering RNA(siRNA)on bladder cancer cells T24 and the sensitivity change of DDP.Methods Three siRNA targeting three different domains of annexin-1 gene were designed,synthesized,and transfected into bladder cancer T24 cells by lipofectamineTM2000.After transfection,expression of annexin-1 was detected by RT-PCR and West-blotting.The cellular sensitivity to DDP was evaluated by MTT assay.Results Compared with the negative control(siRNA-NC)groups,transfection of three targeting siRNA in T24 cells decreased the expression of annexin-1 mRNA and protein.Annexin-1 gene silencing increased the sensitivity of T24 cells to DDP.Conclusions The chemically synthesized specific siRNA targeting annexin-1 could effectively reduce the expression of annexin-1 gene,increase the sensitivity of T24 cells to DDP.

【关键词】 膀胱癌膜联蛋白-1siRNA顺铂
【Key words】 Bladder cancerAnnexin-1siRNADDP
【基金】 黑龙江省科技攻关(国际合作)项目(编号:WB07C06)
  • 【文献出处】 中国老年学杂志 ,Chinese Journal of Gerontology , 编辑部邮箱 ,2010年12期
  • 【分类号】R737.14
  • 【被引频次】1
  • 【下载频次】163
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