【摘要】 目的探讨不同年龄小鼠脑组织中谷胱甘肽(GSH)含量及相关酶活性的差异。方法比较3,8和24周龄小鼠脑组织中GSH含量及GSH合成酶(GCL)、GSH还原酶(GR)、GSH过氧化物酶(GSH-Px)和GSH巯基转移酶(GST)活性的差异。结果不同年龄小鼠脑内GSH含量、GCL和GR活性无明显变化。8和24周龄小鼠脑内GSH-Px明显低于3周龄组。24周龄小鼠脑内GST活力明显高于3和8周龄。结论小鼠脑内GSH含量及其合成相关的GCL和GR活性不随年龄明显改变,而GSH依赖的抗氧化酶GST和GSH-Px的活性则具有明显差异。更多还原

【Abstract】 Objective To investigate the difference of glutathione contents and the activities of glutathione-related enzymes in mice brains of different age.Methods The mice were divided into 3 groups:3,8 and 24 weeks group respectively,and 10 mice in each group.The glutathione contents,and the activities of glutamate cysteine ligase(GCL),glutathione reductase(GR),glutathione peroxidase(GSH-Px),glutathione-S-transferase(GST)were observed in mice brains of different age.Results There were no significant differences in glutathione contents,GCL and GR activities in brains between different age mice.However,the GSH-Px activity was lower in 8 and 24 weeks group than that in 3 weeks group.The GST activity in 24 weeks group was higher than that in 3 and 8 weeks groups.Conclusions The glutathione contents and the activities of GCL and GR,which regulate cellular glutathione synthesis,do not change in mice brains with different age.However,there are significant differences in glutathione-dependent antioxidant enzymes in mice brains of different age,such as GST and GSH-Px.更多还原