【摘要】 目的建立人恶性淋巴瘤细胞Raji长春新碱(VCR)耐药株Raji/VCR并初步研究其生物学特性。方法采用大剂量VCR间歇诱导法建立耐药株Raji/VCR。MTT法测定其对VCR、足叶乙甙(VP-16)和阿霉素(ADM)的药物敏感度,Real-time RT-PCR法测定细胞DMR1 mRNA水平,流式细胞仪检测P-gp表达和细胞内柔红霉素(DNR)浓度。结果历时近8月建成人恶性淋巴瘤多药耐药株Raji/VCR,其对VCR、ADM、VP-16的耐药倍数分别为8、5和3。Raji/VCR的DMR1 mRNA及P-gp表达明显高于亲本细胞,而细胞内DNR浓度明显下降。结论耐药株Raji/VCR具有多药耐药细胞的基本生物学特性,可用于肿瘤多药耐药的相关研究。更多还原

【Abstract】 Objective To establish VCR-resistant cell line (Raji/VCR) from human malignant lymphoma cell line Raji and to initially investigate its bionomics.Methods Pulse exposure to high dose VCR was used to establish resistant cell line Raji/VCR. MTT essay was used to study its drug sensitivity to VCR、VP-16 and ADM. LeveLs of DMR1 mRNA expression were detected by real time reverse transcription-polymerase chain reaction(real time RT-PCR). Intracellular rubidomycin(DNR) concentration and P-glycoprotein(P-gp) were examined by flow cytometry(FCM).Results The human multidrug-resistant malignant lymphoma cell line Raji/VCR was established after 8 months selective culture whose resistance multiple to VCR、ADM、VP-16 were 8,5,3 respectively.The expression of DMR1 mRNA and P-gp in Raji/VCR were significantly higher than that in Raji,but intracellular DNR concentration wgs markly reduced.Conclusion Raji/VCR have the basal bionomics of multidrug-resistant cell lines,and may be used for investigation related to multidrug-resistance of tumor.更多还原