【摘要】 从福州土壤样品中分离出一株能够转化西罗莫司的细菌,编号287,经形态、生理生化特性和16S rRNA基因序列分析鉴定为巨大芽孢杆菌,该菌以西罗莫司为底物,经28℃,72h振荡培养,分离得到3个主要产物:287-P1,287-P2和287-P3。其中,287-P1经结构鉴定为29,42-O-双去甲基雷帕霉素。更多还原

【Abstract】 Bacterium strain 287 isolated from the soil samples in Fuzhou,China was identified as Bacillus megaterium based on the taxonomic properties and 16s rRNA gene sequence.Bacillus megaterium 287 biotransformed sirolimus to three compounds: 287-P1,287-P2 and 287-P3.Among them,287-P1 was identified as 29,42-bis-O-demethylrapamycin by physico-chemical properties and spectral analyses.更多还原