【摘要】 目的研究谷氨酸是否引起培养大鼠星形胶质细胞水通道蛋白4(AQP4)表达变化。方法利用传代培养至10d左右的大鼠星形胶质细胞,给予1mmol/LL-谷氨酸钠,分别作用1h、3h、6h、12h、24h、48h后进行细胞形态学观察、GFAP免疫化学染色及荧光定量PCR检测AQP4mRNA表达变化。结果谷氨酸作用1h后星形胶质细胞出现肿胀并随着作用时间延长肿胀持续,AQP4mRNA3h时开始出现表达降低(P<0.05),后随谷氨酸作用时间延长先下调后上调,12h达最低(P<0.01),48h升至高于正常(P<0.05)。结论AQP4可能在谷氨酸引起星形胶质细胞肿胀发生发展中发挥作用。更多还原

【Abstract】 Objective To determine whether glutamate induces the alteration of aquaporin 4 (AQP4) expression in cultured rat astrocytes.Methods The secondary cultured astrocytes were treated with 1 mmol/L L-glutamate for 1 h,3 h,6 h,12 h,24 h and 48 h.The morphologic changes of astrocytes were observed through microscope after GFAP immunostaining and AQP4 mRNA expression were detected with real-time PCR.Results The astrocytes swelled when exposed to glutamate for 1 h and remained with prolonged treatment.Meanwhile,the AQP4 mRNA expression were early down-regulated and subsequently up-regulated,featured with the lowest AQP4 mRNA level at 12 h after treatment (P<0.01) and higher at 48 h (P<0.05).Conclusion Aquaporin 4 may be involved in the occurrence and development of astrocyte swelling induced by glutamate.更多还原