【摘要】 运用差异显示PCR(DD-PCR)方法分析棉铃虫滞育解除蛹差异表达的基因,结果得到了56个差异片段.通过RT-PCR和Real-time PCR的方法,鉴定了滞育解除过程中有3个基因表达量在下调,有4个基因表达量在上调.这7个差异表达基因中有3个和已知的基因有较高的同源性:FKBP12,esr16和NADH dehydrogenase 1 alpha subcomplex subunit 6.这些差异基因为今后研究滞育解除的分子机制提供了新的线索.更多还原

【Abstract】 By means of mRNA differential display PCR(DD-PCR),56 cDNA fragments have been identified from the Helicoverpa armigeras brain that are expressed differentially between the diapausing pupae and the pupae of diapause termination by injecting 20-hydroxyecdysone.RT-PCR and Real-time PCR analysis revealed that mRNA levels of the FK506-binding protein 12(FKBP12)and two unknown genes were higher in the diapausing pupae,whereas the ecdysteroid-regulated 16 kDa protein(esr16),NADH dehydrogenase 1 alpha subcomplex subunit 6,and two unknown genes were higher in the pupae of diapause termination.These results provide new clues to researches on the molecular mechanism of diapause.更多还原