【摘要】 [目的]观察创伤愈合过程中神经再生的动态变化。[方法]采取伤区组织及瘢痕组织标本,改良三色法染色观察胶原;利用免疫荧光技术以神经丝蛋白作为标记物,免疫荧光显微镜、激光共聚焦扫描显微镜(LSCM)下观察神经纤维的二、三维结构。[结果]伤后胶原比例逐渐增大,到增生期胶原容积分数达到最高,成熟期趋于正常。伤后神经持续再生,瘢痕增生期神经纤维容积分数达到最高,到成熟期神经纤维容积分数则低于正常。三维重建发现增生期神经纤维存在扭曲变形,崩解断裂现象,成熟期则趋于正常。[结论]烧(创)伤愈合过程中神经纤维经历数目逐渐增多,形态出现扭曲粗大,然后经历崩解断裂、数目减少、形态规则的重构过程。更多还原

【Abstract】 [Objective]To observe changes of skin reinnervation during burn wound healing and determine remodeling of nerve fibers.[Method]Wound tissue and scars were harvested.Collagen was determined with modified Masson trichrome staining and nerve fibers were labeled with neuro filament protein by immunofluorescent technique.Three dimension reconstruction of nerve fibers regenerated was observed under laser scanning confocal microscope.[Result]The CVF(collagen volume fraction) increased during the process of wound healing.The regenerated nerve fibers were sparse,short and small,which was significant lower the normal control.The skin reinnervation improved during the wound healing process and came to peak NVF(nerve fiber volume fraction) in proliferative stage.Disintegration and fragmentation were observed frequently in samples from proliferative stage,which seldom occurred during mature stage.[Conclusion]The remodeling of skin nerve fibers comprises a sequential process of increasing number,shape distortion during wound healing and then disintegration,number decrease and normal shape.更多还原