【摘要】 以铝敏感(华油2790,简称华油)和耐性(中双7号,简称中双)油菜品种为材料,在水培条件下,研究了0,50,100和200μmol/L铝胁迫处理7 d后对油菜根和叶的各项生理指标的影响.结果表明:随着铝处理浓度的增加,油菜的相对根长逐渐减小,华油的抑制率大于中双,但两者相对株高的变化不大;根的总长和总表面积等均随着铝处理浓度的增加而减小,但根系直径在200μmol/L铝处理时都略有增加;叶的各项指标则变化不大.此外,各项叶绿素荧光参数,如最大荧光(Fm)、PSⅡ潜在活性(Fv/F0)、光化学量子产量(Yield)等均随着铝浓度的增加而降低,而初始荧光(F0)与上述指标的变化趋势相反,中双的变化幅度小于华油.铝处理下,油菜根尖的酸代谢酶——柠檬酸合成酶(CS)、苹果酸脱氢酶(MDH)、磷酸烯醇式丙酮酸羧化酶(PEPC)和乌头酸酶(ACO)等的活性均高于对照,且随着铝处理浓度的增加而增加.表明铝胁迫对油菜根和叶均造成了一定的伤害,且对根的影响更明显.随着铝浓度的增加,根尖各代谢酶的活性增加,可能是铝胁迫促进了油菜的生理代谢,是对铝毒的一种生理响应机制.更多还原

【Abstract】 Takeing Al-sensitive(Hua You 2790) and Al-tolerant(Zhong Shuang NO.7) varieties of oil rape as materials,in water culture conditions,it was studied the effect of 0,50,100,200 μmol/L Al stress on roots and leaves of the physiological indicators seven days later.The results showed that: with the increase of Al concentration,the relative root length gradually reduced,Hua You 2790 was greater than Zhong Shuang NO.7,but there was little change in relative height.Total root length,total surface area et al.all decreased with the increase of of Al concentration,while root diameter had a slight increase in 200 μmol/L,but it did not show significant differences in both leaf indexes.In addition,the chlorophyll fluorescence parameters,such as Fm(maximal fluorescence),Fv/F0(PSⅡ potential activity),Yield(photochemical quantum yield) and others all decreased with the increasing concentration of Al,while the F0(initial fluorescence) was just in the opposite.The magnitude of changes in Zhong Shuang NO.7 were less than Hua You 2790.Under Al treatment,the tip of the acid-metabolizing enzymes: such as CS(citrate synthase),MDH(malate dehydrogenase),PEPC(phosphoenolpyruvate carboxylase),ACO(aconitase),their activity were higher compared with the control,and increased with the increase of Al concentration.As a result,Al stress on roots and leaves of oil rape caused a certain amount of damage,and an even greater impact on the root;with the increase of Al concentration,the metabolic activity of the enzymes in root tip increased,which may be contributed to the physiology metabolism of rape under aluminum stress,and it was a physiological response mechanism to Al toxicity.更多还原