【摘要】 在基础饲料中分别添加0(对照组),1%,2%,3%小肽,研究小肽对凡纳滨对虾(Penaeus vannamei)幼体生长和非特异性免疫力的影响。60 d后测定凡纳滨对虾的生长性能及血清抗菌活力、溶菌酶和酚氧化酶活性,并用哈维氏弧菌进行攻毒试验。结果表明,小肽能显著提高凡纳滨对虾的生长性能(P<0.05),1%,2%和3%处理组体长增长率分别比对照组增加了17.74%,27.75%和17.46%,体重增长率分别比对照组增加了12.15%,31.16%和19.01%;饲料系数分别比对照组下降了21.57%,33.99%和25.49%;成活率分别比对照组提高了12.23%,18.89%和8.89%。小肽能显著增强凡纳滨对虾的非特异性免疫能力,1%,2%,3%处理组抗菌活力分别比对照组提高了21.05%,42.10%,10.53%;血清溶菌酶比活力分别比对照组提高了16.54%,24.56%,15.54%;酚氧化酶活力分别比对照组提高了25.70%,41.90%,24.02%。哈维氏弧菌攻毒试验结果表明,5 d内对照组累计死亡率为96.7%,高于各处理组,但差异不显著;综合试验结果得知,饲料中添加小肽能显著提高凡纳滨对虾生长性能和血清非特异性免疫能力,2%为适宜添加量。更多还原

【Abstract】 The present study was conducted to determine the effect of small peptides on growth performance and non-specific immune of Penaeus vannamei.Four isonitrogenous and isoenergetic experimental diets were formulated.Diet containing no small peptides served as the control diet.The other three trial diets was added 1%,2%,3% small peptides respectively.After 60 d-feeding,growth rate,serum antibacterial activity,lysozyme activity and phenol oxidase(PO) activity were measured,a challenge test was conducted with Vibrio harveyi.The results showed that there was significant difference in length gain,weight gain,feed coefficient and survival rate(P<0.05) of Penaeus vannamei among the control group and the treatments.The length gain of 1%,2% and 3% trial group was 17.74%,27.75% and 17.46% higher than that of the control,respectively.The weight gain of shrimp was 12.15%,31.16% and 19.01% higher than that of the control,respectively.The feed coefficient was 21.57%,33.99% and 25.49% lower than that of the control,respectively.The survival rate was 12.23%,18.89% and 8.89% higher than that of the control,respectively.Small peptides significantly enhanced(P<0.05)non-specific immune activity of Penaeus vannamei.The antibacterial activity of 1%,2% and 3% trial group were 21.05%,42.10% and 10.53% higher than that of the control,respectively.The lysozyme activity were 16.54%,24.56% and 15.54%,PO activity were 25.70%,41.90% and 24.02% higher than that of the control,respectively.The results of Vibrio harveyi challenge showed that cumulative mortality of the control in the five days was 96.7% and higher than that of the trial groups.It was concluded that the upper limit of small peptides for optimal growth performance and non-specific immune function in Penaeus vannamei feed was about 2%.更多还原