【摘要】 目的通过检测河南省0～12岁健康儿童、手足口病痊愈病人、手足口病现症病人血清肠道病毒71型(EV71)抗体水平,了解手足口病优势病毒株EV71人群感染情况,为预防控制手足口病等提供科学依据。方法在河南省不同地区采集0～12岁健康儿童、手足口病痊愈病人和手足口病现症病人血清共226份,采用微量中和滴定法检测抗EV71抗体。结果健康儿童、手足口病痊愈病人和手足口病现症病人血清EV71中和抗体阳性率分别为51.46%、92.96%和55.77%,差异有统计学意义(P<0.05),EV71几何平均滴度(GMT)分别为11.13(8.83～13.99)、66.51(52.09～84.97)和21.93(14.32～33.57),差异有统计学意义(P<0.05)。结论手足口病流行地区健康儿童血清EV71中和抗体阳性率较高,EV71阴性感染较普遍。手足口病康复病人能够产生较高滴度的免疫保护性抗体,对EV71病毒再次感染具有一定的保护作用。更多还原

【Abstract】 Objective To study the status of antibodies with respect to recently circulating enterovirus 71(EV71) strains in healthy children 0-12 years old,individuals who have recovered from hand,foot,and mouth disease,and children with hand,foot,and mouth disease in order to provide scientific evidence for prevention and control of EV71 causing hand,foot,and mouth disease.Methods A microneutralization test was used to study 226 serum samples collected from sera of different populations in Henan Province,including healthy children,individuals who have recovered from hand,foot,and mouth disease,and children with hand,foot,and mouth disease.Results Anti-EV71 antibody was detected at respective rates of 51.46%,92.96%,and 55.77% in healthy children,individuals who have recovered from hand,foot,and mouth disease,and children with hand,foot,and mouth disease.The rates at which antibodies were detected differed significantly for all the groups.The geometric mean of the antibody titer(GMT) in healthy children,individuals who have recovered from hand,foot,and mouth disease,and children with hand,foot,and mouth disease was 11.13(8.83-13.99),66.51(52.09-84.97),and 21.93(14.32-33.57),respectively.The GMT differed significantly among the three groups but was significantly higher for individuals who have recovered from hand,foot,and mouth disease than for healthy children and children with hand,foot,and mouth disease.Conclusion The positive rate of anti-EV71 antibody detection increased significantly for healthy children in areas where hand,foot,and mouth disease is prevalent.Individuals who have recovered from hand,foot,and mouth disease have robust antibody titers,which may protect them from reinfection with EV71.更多还原