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日本血吸虫不同发育阶段形态与生长速度差异分析
Analyzing differences in growth rates of Schistosoma japonicum worms at various points in development
【摘要】 目的观察日本血吸虫不同发育阶段的生长速度差异。方法采用清洁级昆明鼠,经人工感染日本血吸虫尾蚴,分别收集感染前0 d和感染后第8、12、16、20、24和28 d的虫体,用Motic BA400病理图像处理系统及3.2分析软件采集虫体图象,作形态观察和多指标测量。结果发育中的童虫在不同阶段生长速度不均衡,以8~12 d时生长最快,其他时段依次为12~16 d、16~20 d、20~24 d、0~8 d和24~28 d;同一阶段的虫体发育差异较大,尤其在12 d后。结论日本血吸虫童虫在不同发育时段的生长速度存在明显差异;8~12 d时虫体生长最快。
【Abstract】 Objective To observe the differences in growth rates of Schistosoma japonicum(Sj)at different points in development.Methods Worms were recovered from Kunming mice infected with Sj cercariae on days 0,8,12,16,20,24,and 28 post-infection,respectively.Pathological Image Processing System Motic BA400 and 3.2 software were utilized to observe morphology and measure worm area,perimeter,length,and width.Results Worms at different points in development grew at distinctive rates.Worms on days 8 to 12 had the fastest development,followed by worms on days 12 to 24,0 to 8,and 24 to 28.Even at the same point in time,there were obvious distinctions among the worms,especially after day 12.Conclusion The growth rate of Sj worms differs at different points in the worms’ development.The growth rate of 8 to 12-day old worms was the fastest.
【Key words】 Schistosoma japonicum; points in development; morphology; growth rate;
- 【文献出处】 中国病原生物学杂志 ,Journal of Pathogen Biology , 编辑部邮箱 ,2010年06期
- 【分类号】R383.24
- 【被引频次】3
- 【下载频次】216