【摘要】 目的调查云南省景东县流行性乙型脑炎的流行现状。方法媒介蚊虫调查采用诱蚊灯通宵捕蚊方法,蚊虫乙脑病毒检测采取RT-PCR方法,健康人群血清乙脑病毒抗体检测采用ELISA。结果共采集到蚊虫4属15种6653只,其中三带喙库蚊和中华按蚊属于当地的优势种群,分别占总捕获蚊数的82.07%和10.46%。RT-PCR检测三带喙库蚊和中华按蚊共16批1310只,各有1株感染乙脑病毒;ELISA检测当地健康人群血清乙脑病毒抗体阳性率为72.96%,不同年龄组人群乙脑病毒抗体水平差异有统计学意义(χ2=51.589,P<0.01)。结论云南景东县蚊虫种类较多,三带喙库蚊和中华按蚊有传播乙型脑炎的作用。更多还原

【Abstract】 Objective To explore the prevalence of Japanese encephalitis(JE) in Jingdong County,Yunnan Province.Methods Mosquito lamp traps were used to capture mosquitoes overnight.RT-PCR was used to detect the virus in mosquitoes infected with the Japanese encephalitis virus(JEV),and enzyme-linked immunosorbent assay(ELISA) was used to detect JEV antibodies in healthy residents.Results A total of 6 653 individual mosquitoes from 15 species in 4 genera were collected.Of these mosquito species,Culex tritaeniorhynchus and Anopheles sinensis were the local dominant species,and they accounted for 82.07% and 10.46% of the total mosquitoes collected.RT-PCR detected a JEV strain in both Cx.tritaeniorhynchus and An.sinensis in 16 batches of 1 310 individual mosquitoes.ELISA detected JEV antibodies at a rate of 72.96% from the sera of healthy residents,and there was a significant difference between age groups(χ2=51.589,P<0.01).Conclusion There are a relatively large number of vectors of Japanese encephalitis in Jingdong County,and Cx.tritaeniorhynchus and An.sinensis play an important roles in local JE transmission.更多还原