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抗生素降低彩色马蹄莲组织培养污染的研究

Study on the Effect of Several Antibiotics in Reducing Pollution of Zantedeschia Hvbrida Tissue Culture

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【作者】 周凤娇巫华美陈训

【Author】 ZHOU Feng-Jiao1,WU Hua-mei2,CHEN Xun3(1.Forestry Research Institute of Guizhou Province,Guiyang 550005,China;2.Institute of Biology,Guizhou Province,Guiyang 550009,China;3.Guizhou Academy of Sciences,Guiyang 550001,China)

【机构】 贵州省林业科学研究院贵州省生物研究所贵州科学院

【摘要】 以生长于土壤中易污染的马蹄莲一年生小块茎为试材,在培养基中添加硫酸庆大霉素、青霉素、硫酸链霉素3种抗生素,浓度梯度为25、50 mg/L和75 mg/L进行初代培养进行组织培养,未添加抗生素作为对照。结果表明:3种抗生素都可以明显降低污染率,而以硫酸链霉素抑菌效果最好。特别是浓度为50 mg/L的链霉素抑菌效果最明显。

【Abstract】 Some small tubers of annual of Zantedeschia,growing in the soil pollution were cultured in the medium of added Gentamicin,penicillin,streptomycin,which concentration gradient were 25mg/L,50mg/L and 75mg/L,without addition of antibiotics as control.The results showed that Three kinds of antibiotics could significantly reduce the contamination rate,while the inhibitory effect of streptomycin sulfate was the best,especially in 50mg/L treatment.

【关键词】 抗生素彩色马蹄莲初代培养污染
【Key words】 antibioticsZantedeschia hvbridaprimary culturepollution
【基金】 贵州省农业科技攻关项目[黔科合NY字(2006)3027号]
  • 【分类号】S682.264
  • 【被引频次】5
  • 【下载频次】213
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